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In vivo Quantification of G Protein Coupled Receptor Interactions using Spectrally Resolved Two-photon Microscopy

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Tuning Polyacrylate Composition to Recognize and Modulate Fluorescent Proteins.

Darwin C Gomez^1,2,3, Swarnadeep Seth⁴, Ronnie Mondal¹

¹Macromolecules Innovation Institute and Department of Chemistry, Virginia Tech, Blacksburg, Virginia, 24061, USA.

Angewandte Chemie (International Ed. in English)

|November 17, 2025

Summary

This summary is machine-generated.

Synthetic polymers, termed random hetero oligomers (RHOs), can selectively bind to proteins like green fluorescent protein (GFP) without specific sequences. This discovery opens doors for advanced materials in sensing and therapeutics.

Keywords:

Copolymers Polymer synthesis Polymer–protein conjugate Protein RAFT

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Area of Science:

Polymer Chemistry
Biomaterials Science
Molecular Recognition

Background:

Molecular definition is typically required for protein recognition and functional modulation.
Macromolecular interactions often depend on precise molecular structures.

Purpose of the Study:

To investigate if polymers with random monomer sequences can achieve selective protein binding.
To explore the potential of synthetic polymers as platforms for molecular recognition.

Main Methods:

Development of polyacrylates as random hetero oligomers (RHOs).
Utilizing green fluorescent protein (GFP) as a model target protein.
Affinity and selectivity assays, including competition experiments.
Experimental and computational analyses of RHO-GFP interactions.

Main Results:

RHOs demonstrated selective binding to GFP with nanomolar affinity.
GFP fluorescence was enhanced by over 100% upon binding.
Purified RHO subpopulations exhibited distinct affinities and selectivity.
RHO-protein interactions were confirmed to be influenced by RHO chemical composition.

Conclusions:

Sequence-defined structures are not essential for selective protein recognition.
Synthetic polymers offer scalable and tunable platforms for molecular recognition.
This advancement paves the way for next-generation sensing, therapeutic, and catalytic materials.