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Published on: March 29, 2012
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Proteomic Insights into Small Molecule Modulation on Caudal Fin Regeneration in Zebrafish
P V Anusha1, Shailvi Tewari1, Catherine Philip1
1CSIR-CCMB, Uppal Road, Hyderabad 500 007, India.
ACS Omega
|November 17, 2025
Summary
Small molecules like ethanol, glucose, and NaCl impair zebrafish fin regeneration and cause behavioral changes. Proteomic analysis revealed common molecular responses to stress and repair across treatments, impacting key signaling pathways.
Area of Science:
- Regenerative Biology
- Molecular Toxicology
- Zebrafish Models
Background:
- Small molecules significantly influence in vivo conditions and cellular processes vital for tissue regeneration.
- Zebrafish are a robust model for studying regenerative growth due to their appendage regrowth capabilities.
Purpose of the Study:
- To systematically evaluate the impact of ethanol, glucose, and NaCl on zebrafish caudal fin regeneration.
- To investigate the molecular and behavioral responses to these small molecule perturbations.
Main Methods:
- Zebrafish caudal fin regeneration assay over 7 days.
- Behavioral assessments for locomotor activity.
- Proteomic profiling using label-free quantification and iTRAQ labeling.
- Pathway enrichment analysis.
Main Results:
- All tested small molecules delayed fin regrowth, with glucose showing the most significant impairment.
- Ethanol exposure led to the most pronounced reduction in locomotor activity.
- Proteomic analysis identified differentially expressed proteins and common molecular responses related to stress and repair, implicating pathways like GP6 signaling and mitochondrial dysfunction.
Conclusions:
- Metabolic and ionic perturbations disrupt zebrafish fin regeneration and induce stress responses.
- Understanding these molecular mechanisms provides insights into tissue repair under adverse conditions.
