Physical and Chemical Modifications of Polymeric Surfaces to Enhance Epithelial Cell Adhesion

In tissue engineering, 3D scaffolds and chemical treatments are often used to provide a cell-friendly surface that improves cell adhesion and tissue growth. Indeed, cell adhesion can be regulated through physicochemical modifications of the substrate surface, including wettability, surface charge, and roughness. In this work, we present the synthesis, characterization, and cytocompatibility analysis of photoresins suitable for fabricating cell scaffolds via two-photon polymerization. Furthermore, we demonstrate a simple surface treatment method that promotes cell adhesion. This method alters the surface charge of the polymer and enhances the adhesion of epithelial cells. Our results indicate an efficient approach to modifying the surface of biocompatible polymer scaffolds with the purpose of enhancing the performance of cell functions that are suitable for tissue engineering and regenerative medicine.