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Related Experiment Video

Updated: Jul 21, 2026

Dissection and Immunohistochemistry of Larval, Pupal and Adult Drosophila Retinas
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Dicer is essential for proper maturation, composition, and function in the postnatal retina.

Seoyoung Kang1, Daniel Larbi1, Eik Bruns1

  • 1Department of Biological and Vision Sciences, The State University of New York, College of Optometry, New York, NY, USA.

Iscience
|November 17, 2025
PubMed
Summary

Dicer and microRNAs are crucial for proper retinal development after birth. Depleting Dicer in retinal cells impairs vision and alters cell populations in adult mice, indicating a vital role in cell maturation.

Keywords:
Molecular biologyNeurosciencecell biologydevelopmental biology

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Area of Science:

  • Ophthalmology
  • Developmental Biology
  • Molecular Biology

Background:

  • The role of Dicer and microRNAs (miRNAs) in postnatal retinal development remains largely unexplored.
  • Dicer is essential for miRNA biogenesis, which regulates gene expression post-transcriptionally.

Purpose of the Study:

  • To investigate the impact of Dicer depletion in postnatal retinal progenitor/precursor cells (RPCs/PCs) on retinal structure and function.
  • To characterize the adult Dicer-conditional knockout (cKO) retina.

Main Methods:

  • Conditional knockout of Dicer in RPCs/PCs.
  • Evaluation of adult Dicer-cKO retina using optical coherence tomography (OCT), electroretinography (ERG), and histology.
  • Transcriptional analysis (mRNA and miRNA levels) and single-cell RNA sequencing in young mice.

Main Results:

  • Impaired rod function and reduced rod bipolar cell numbers and function.
  • Decreased Müller glia population and increased amacrine cell population (HuC/D+ Pax6+).
  • Early alterations in mRNA and miRNA levels, with delayed differentiation and persistent enlarged progenitor/precursor populations into adulthood.

Conclusions:

  • Dicer/miRNAs in late RPCs/PCs are essential for the proper formation and maturation of their progeny during postnatal retinogenesis.
  • Dicer/miRNAs play a significant role in regulating cell state and differentiation timing in the developing retina.