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Related Experiment Video

Updated: Jan 11, 2026

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
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Multiplex PCR-based rapid pathogen identification in acute cholecystitis using the FilmArray BCID2 panel.

Gaku Nakamura1,2, Koji Asai1, Ryutaro Watanabe1

  • 1Department of Surgery, Toho University Ohashi Medical Center, Tokyo, Japan.

Frontiers in Microbiology
|November 17, 2025
PubMed
Summary
This summary is machine-generated.

Rapidly identifying bacteria in acute biliary infections using the FilmArray® Blood Culture Identification 2 (BCID2) panel improves diagnostic accuracy. This multiplex PCR test aids in timely, targeted antimicrobial therapy for better patient outcomes.

Keywords:
acute biliary infectionacute cholecystitisantimicrobial resistanceblood culture identification 2multiplex PCR system

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Area of Science:

  • Microbiology
  • Infectious Diseases
  • Molecular Diagnostics

Background:

  • Acute biliary infections pose significant clinical challenges, with delayed treatment potentially leading to adverse outcomes and antimicrobial resistance.
  • Accurate and rapid pathogen identification is crucial for effective management of these serious infections.

Purpose of the Study:

  • To evaluate the performance of the FilmArray® Blood Culture Identification 2 (BCID2) panel for rapid pathogen detection in bile samples from patients with acute cholecystitis.
  • To assess the utility of multiplex PCR in improving diagnosis and guiding therapy for biliary tract infections.

Main Methods:

  • Bile samples from 77 patients with acute cholecystitis were analyzed using both conventional culture and the BCID2 multiplex PCR panel.
  • Conventional culture served as the reference standard to determine the accuracy of the BCID2 panel, calculating metrics like positive percent agreement (PPA) and negative percent agreement (NPA).

Main Results:

  • The BCID2 panel demonstrated high accuracy (92.2%) in identifying pathogens directly from bile samples.
  • Sensitivity (PPA) for detecting targetable isolates was 82.4%, with 100% specificity (NPA).
  • Detection rates were higher in polymicrobial infections (94.1%) and with increased bacterial load (>10^6 CFU/mL).

Conclusions:

  • Multiplex PCR testing with the BCID2 panel offers a rapid and accurate method for identifying bacterial pathogens in bile.
  • This molecular approach can facilitate earlier targeted antimicrobial therapy and support antimicrobial stewardship in acute biliary infections.