Related Experiment Video
Updated: Jan 11, 2026
Establishing Cell Lines Overexpressing DR3 to Assess the Apoptotic Response to Anti-mitotic Therapeutics
Published on: January 11, 2019
Apoptotic effect of linifanib on human ovarian cancer OVCAR3 cell line
Han Ki Lee1, Jongsung Lee2, Myeong Jin Nam1
1Department of Biological Science, Gachon University, Seongnam 13120, Republic of Korea.
Background And Purpose:
Linifanib is a multi-targeted receptor tyrosine kinase inhibitor. Although it is widely recognized for its efficacy in inhibiting receptor tyrosine kinases, its anticancer effects in ovarian cancer have not been extensively studied. In this study, we investigated the anticancer effects of linifanib on human ovarian cancer OVCAR3 cells.
Experimental Approach:
To evaluate cell proliferation capacity, we performed MTT assays, cell counting, morphological analysis, and colony formation assays. Flow cytometry was used to assess the induction of apoptotic cells by linifanib, and DNA fragmentation was evaluated using TUNEL assays. Western blotting was performed to determine the protein expression levels related to apoptosis.
Findings/Results:
Our results demonstrated that linifanib significantly inhibited the proliferation of OVCAR3 cells and induced apoptosis. Notably, treatment with linifanib led to the inhibition of phosphorylated Akt at Ser473, accompanied by the activation of FOXO3.
Conclusion And Implications:
Taken together, these findings indicate that linifanib suppresses the proliferation of human ovarian cancer OVCAR3 cells, highlighting its potential as a therapeutic candidate for ovarian cancer treatment.
Insights
Linifanib, a receptor tyrosine kinase inhibitor, effectively suppressed ovarian cancer cell proliferation and induced apoptosis in OVCAR3 cells. This study highlights linifanib
Area of Science:
- Oncology
- Molecular Biology
- Pharmacology
Background:
- Linifanib is a multi-targeted receptor tyrosine kinase inhibitor.
- Its anticancer effects in ovarian cancer require further investigation.
- Ovarian cancer remains a significant health concern globally.
Purpose of the Study:
- To investigate the anticancer effects of linifanib on human ovarian cancer OVCAR3 cells.
- To evaluate linifanib's impact on cell proliferation and apoptosis.
- To explore the underlying molecular mechanisms of linifanib's action.
Main Methods:
- MTT assays, cell counting, and colony formation assays were used to assess proliferation.
- Flow cytometry and TUNEL assays evaluated apoptosis induction.
- Western blotting analyzed protein expression related to apoptosis, including Akt and FOXO3.
Main Results:
- Linifanib significantly inhibited OVCAR3 cell proliferation.
- Linifanib treatment induced apoptosis in ovarian cancer cells.
- Inhibition of phosphorylated Akt (Ser473) and activation of FOXO3 were observed.
Conclusions:
- Linifanib demonstrates potent antiproliferative and pro-apoptotic effects on human ovarian cancer cells.
- The study identifies inhibition of the Akt/FOXO3 pathway as a key mechanism.
- Linifanib shows promise as a potential therapeutic agent for ovarian cancer.
Related Concept Videos
01:31The Intrinsic Apoptotic Pathway
01:20The Retinoblastoma Gene
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...