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Multiphoton microscopy imaging of fibrous meningiomas based on the combination of multichannel mode and lambda mode
Linghan You1, Linjing Shi1, Yuqing Huang1
1School of Medical Technology and Engineering, Fujian Medical University, Fuzhou, China.
Abstract:
Fibrous meningiomas, known for their dense and tough texture, present unique challenges in diagnosis and surgical treatment. This study explores the potential of multiphoton microscopy (MPM) for visualizing the microstructures of fibrous meningiomas by combining multichannel and lambda modes. Using MPM, we imaged 14 fibrous meningioma samples collected from neurosurgical procedures. The multichannel mode captured second harmonic generation (SHG) and two-photon excitation fluorescence (TPEF) signals, while the lambda mode provided detailed spectral imaging across 32 channels. Image analysis algorithms were developed to quantify collagen content and assess morphological features. Spectroscopic analysis revealed the intrinsic components of fibrous meningiomas, with collagen being the most abundant component (relative ratio: 0.952), followed by structural proteins (0.502), free-form NADH (0.393), FAD (0.199), lipopigments (0.198), protein-bound NADH (0.105), porphyrin derivatives I (0.104), and porphyrin derivatives II (0.015). The combined spectral images also provided high-contrast and high-resolution views of the tumor microenvironment. Quantitative analysis showed that the average collagen content in fibrous meningioma tissues was 0.537 ± 0.131 using SHG imaging and 0.503 ± 0.133 using combined 32-channel spectral imaging. With the advancement of fiber optic technology and multiphoton endoscopy, multiphoton microscopy holds promise as a new technology for clinically diagnosing fibrous meningiomas.
Insights
Multiphoton microscopy (MPM) visualizes fibrous meningioma microstructures, revealing collagen as the main component. This advanced imaging technique shows promise for improved clinical diagnosis of these challenging tumors.
Area of Science:
- Neuroscience
- Biomedical Imaging
- Oncology
Background:
- Fibrous meningiomas are challenging to diagnose and treat due to their dense texture.
- Current diagnostic methods may not fully capture the microstructural details of these tumors.
Purpose of the Study:
- To explore the utility of multiphoton microscopy (MPM) for visualizing fibrous meningioma microstructures.
- To combine multichannel and lambda modes of MPM for enhanced imaging and spectral analysis.
- To quantify collagen content and assess morphological features for diagnostic insights.
Main Methods:
- Utilized MPM on 14 fibrous meningioma samples.
- Employed multichannel mode for Second Harmonic Generation (SHG) and Two-Photon Excitation Fluorescence (TPEF) signals.
- Applied lambda mode for 32-channel spectral imaging and developed image analysis algorithms.
Main Results:
- Spectroscopic analysis identified collagen as the most abundant component (relative ratio: 0.952).
- Quantitative analysis showed high collagen content (average 0.537 ± 0.131 via SHG).
- Combined spectral imaging provided high-contrast, high-resolution views of the tumor microenvironment.
Conclusions:
- Multiphoton microscopy effectively visualizes fibrous meningioma microstructures and composition.
- MPM offers potential as a novel technology for the clinical diagnosis of fibrous meningiomas.
- Advancements in fiber optics and endoscopy may facilitate clinical integration of MPM.
