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Updated: Jan 11, 2026
Molecular Profiling of the Invasive Tumor Microenvironment in a 3-Dimensional Model of Colorectal Cancer Cells and Ex vivo Fibroblasts
Published on: April 29, 2014
Single-cell and spatial transcriptomics integration reveals FAM49B promotes tumor-associated macrophages polarization
Tianyu Liu1, Quchen Ding2, Jin Gou1
1Department of General Surgery, Sir Run Run Hospital, Nanjing Medical University, Nanjing, Jiangsu, China.
Objectives:
FAM49B has been shown to promote proliferation and metastasis of colorectal cancer (CRC) by stabilizing MYC through phosphorylation of NEK9; however, its role in shaping the immune suppressive tumor microenvironment (TME), particularly in macrophage polarization, remains unclear.
Methods:
We applied multi-omics approaches to study CRC by integrating 33 scRNA-seq samples from 16 CRC patients, 2 paired spatial transcriptomics (ST) samples, and bulk RNA data to characterize malignant epithelial cells (High_FAM49B_EP) and tumor-associated macrophages (TAMs). Functional validation of FAM49B was conducted via knockdown experiments and proteomics analysis.
Results:
A High_FAM49B_EP subpopulation was identified in primary tumors (PT) and liver metastases (LM), exhibiting elevated MYC signaling and association with poor prognosis. TAMs showed spatial heterogeneity: M1-like CXCL3+ TAMs predominated in PT, whereas M2-like SPP1+ TAMs were enriched in LM. CellChat analysis revealed that High_FAM49B_EP activated macrophage polarization through the MDK-NCL signaling axis. Pseudotime trajectory analysis confirmed differentiation from CXCL3+ to SPP1+ TAMs driven by upregulation of NCL. Spatial mapping showed co-localization of MDK+ epithelial cells with NCL+ TAMs in the immunosuppressive microenvironment. FAM49B knockdown significantly inhibited MDK expression and disrupted ECM-receptor interactions.
Conclusions:
FAM49B promotes immunosuppressive TME formation by mediating TAM polarization via the MDK-NCL axis, suggesting the FAM49B-MDK-NCL pathway as a potential therapeutic target for CRC metastasis.
