Functional insights into JrWOX5: a WOX transcription factor regulating adventitious rooting and plant architecture in
Rui-Xue Gao1, Pu Zhang1, Ying-Ying Chang2
1State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Silviculture of National Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091, China.
Key Message:
JrWOX5 promotes adventitious root formation and modulates plant architecture by interacting with key developmental regulators, providing novel insights into WOX-mediated organogenesis in woody plants. WUSCHEL-related homeobox (WOX) transcription factors, a plant-specific gene family, play essential roles in regulating plant development, including stem cell maintenance and organogenesis. Among the WOX genes identified in Juglans regia, JrWOX5 exhibited significantly elevated expression during AR formation, suggesting a potential regulatory role in this process. To investigate its function, we employed a combination of bioinformatics analysis, subcellular localization, heterologous overexpression, yeast two-hybrid (Y2H) assays, and bimolecular fluorescence complementation (BiFC). The JrWOX5 protein was localized to the nucleus. Ectopic expression of JrWOX5 in transgenic poplar markedly promoted AR formation and altered plant architecture, characterized by increased lateral branching and reduced plant height. To elucidate the molecular mechanism, protein-protein interaction (PPI) network analysis was conducted, and key candidate interactors were experimentally validated. JrWOX5 was found to interact with JrLBD16, a LOB domain-containing protein; JrLHW, a transcription factor; and JrCNR8, a regulator of cell proliferation. These findings indicate that JrWOX5 interacts with developmental regulators, which may jointly affect organogenesis and plant architectural patterning. This study aims to provide a new insight into WOX-associated organogenesis in woody plants.
More Related Videos
09:54The Use of Induced Somatic Sector Analysis ISSA for Studying Genes and Promoters Involved in Wood Formation and Secondary Stem Development
Published on: October 5, 2016
09:27High Resolution Quantification of Crystalline Cellulose Accumulation in Arabidopsis Roots to Monitor Tissue-specific Cell Wall Modifications
Published on: May 10, 2016
Related Concept Videos
Meristems and Plant Growth
Canonical Wnt Signaling Pathway
Primary and Secondary Growth in Roots and Shoots
Gene Regulation During Sporulation
Non-Canonical Wnt Signaling Pathways
Cell Signaling in Plants
