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Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....

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Updated: May 6, 2026

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR

Published on: November 28, 2016

Rapid respiratory cryptococcosis detection using targeted next-generation sequencing.

Chaowen Deng¹, Miaoling Qiu², Qingyan Yang³

¹Department of Infectious Diseases and Microbiology, The University of Hong Kong-Shenzhen Hospital, Shenzhen, Guangdong, China.

Plos Neglected Tropical Diseases

|November 17, 2025

Summary

This summary is machine-generated.

Targeted next-generation sequencing (tNGS) offers superior sensitivity and faster diagnosis for pulmonary cryptococcosis compared to traditional methods. This advanced molecular technique aids in detecting infections missed by serum antigen tests, improving patient outcomes.

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Last Updated: May 6, 2026

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR

Published on: November 28, 2016

Amplicon Sequencing using the Long-Read Sequencing Technologies

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Published on: August 29, 2025

Detection of Targetable Alterations in Non-small Cell Lung Cancer using Next-generation Sequencing

Detection of Targetable Alterations in Non-small Cell Lung Cancer using Next-generation Sequencing

Published on: October 10, 2025

Area of Science:

Medical Mycology
Infectious Diseases
Molecular Diagnostics

Background:

Cryptococcus neoformans is a critical-priority fungal pathogen causing cryptococcosis, often leading to fatal meningitis due to diagnostic delays.
Current diagnostic methods like fungal culture and serum cryptococcal antigen (CrAg) testing have limitations in sensitivity and speed.

Purpose of the Study:

To evaluate the clinical utility of targeted next-generation sequencing (tNGS) for diagnosing pulmonary cryptococcosis using respiratory specimens.
To compare the diagnostic performance and turnaround time of tNGS against fungal culture and serum CrAg testing.

Main Methods:

A retrospective study involving 39 patients with confirmed cryptococcosis over 38 months.
Analysis of respiratory specimens using tNGS, fungal culture, and serum CrAg testing.

Main Results:

tNGS detected C. neoformans in 92.3% of respiratory samples, significantly outperforming fungal culture (23.1%).
tNGS identified the pathogen in 12 CrAg-negative and 9 culture-negative cases, enhancing diagnostic yield.
The median turnaround time for tNGS (23 hours) was substantially shorter than for fungal culture (112 hours).
tNGS detected polymicrobial infections in 76.9% of cases.

Conclusions:

tNGS of respiratory specimens offers superior sensitivity and a markedly shorter turnaround time for cryptococcosis diagnosis compared to fungal culture.
tNGS enables earlier diagnosis in CrAg-negative cases, improving detection rates for this neglected fungal pathogen.