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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Updated: Jan 11, 2026

Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry

Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry

Published on: March 17, 2015

Quantification and characterization of extracellular vesicles by flow cytometry.

Ekaterina Petrovich-Kopitman¹, Joshua A Welsh², Shimrit Adutler-Lieber¹

¹Department of Life Sciences Core Facilities, Weizmann Institute of Science, Rehovot, Israel.

Current Topics in Membranes

|November 17, 2025

Summary

Flow cytometry offers a scalable method for analyzing extracellular vesicles (EVs), despite their small size. Optimizations in instrument settings and protocols are key for accurate EV characterization using this versatile technique.

Keywords:

Cell sorting Extracellular vesicles Flow cytometry Imaging flow cytometry

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Last Updated: Jan 11, 2026

Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry

Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry

Published on: March 17, 2015

Flow Cytometric Analysis of Extracellular Vesicles from Cell-conditioned Media

Flow Cytometric Analysis of Extracellular Vesicles from Cell-conditioned Media

Published on: February 12, 2019

Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry

Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry

Published on: July 26, 2022

Area of Science:

Biotechnology
Nanotechnology
Cell Biology

Background:

Extracellular vesicles (EVs) are crucial in cell communication but challenging to characterize due to their small size and heterogeneity.
Current analytical methods often struggle with the comprehensive analysis of EVs.

Purpose of the Study:

To outline the principles, technical considerations, and advantages of using flow cytometry for extracellular vesicle (EV) research.
To highlight the application of flow cytometry and imaging flow cytometry in scientific and clinical contexts.

Main Methods:

Discusses the adaptation of conventional flow cytometry for detecting and analyzing nano-sized EVs.
Emphasizes the importance of instrument optimization, sample handling, labeling strategies, and acquisition protocols.
Highlights the necessity of robust calibration and controls for data accuracy.

Main Results:

Flow cytometry provides a versatile, scalable, and high-throughput platform for multiparametric phenotyping and quantitative detection of EVs.
Specific optimizations enable reliable detection and analysis of smaller EV populations with weaker signals.
Imaging flow cytometry offers advanced capabilities for EV characterization.

Conclusions:

Flow cytometry is a powerful tool for advancing extracellular vesicle (EV) research, offering significant advantages in scalability and throughput.
Optimized protocols and proper controls are essential for reproducible and accurate EV analysis.
The application of flow cytometry in scientific and clinical settings holds great promise for the field.