Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Neurulation01:30

Neurulation

Neurulation is the embryological process which forms the precursors of the central nervous system and occurs after gastrulation has established the three primary cell layers of the embryo: ectoderm, mesoderm, and endoderm. In humans, the majority of this system is formed via primary neurulation, in which the central portion of the ectoderm—originally appearing as a flat sheet of cells—folds upwards and inwards, sealing off to form a hollow neural tube. As development proceeds, the anterior...
Neurogenesis and Regeneration of Nervous Tissue01:15

Neurogenesis and Regeneration of Nervous Tissue

In the CNS, neurogenesis, the birth of new neurons from stem cells, is limited to the hippocampus in adults. In other regions of the brain and spinal cord, neurogenesis is almost non-existent due to inhibitory influences from neuroglia, especially oligodendrocytes, and the absence of growth-stimulating cues. The myelin produced by oligodendrocytes in the CNS inhibits neuronal regeneration. Furthermore, astrocytes proliferate rapidly after neuronal damage, forming scar tissue that physically...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

ZnO@PDA@Ag nanocomposite-mediated delivery of 9-bromonoscapine, an anticancer agent, for enhanced lung cancer therapy.

Nanoscale advances·2026
Same author

Fabrication of a Multifunctional CoFe<sub>2</sub>O<sub>4</sub>@BSA@Ag Nanocomposite: A Novel Biocompatible Nanoplatform for Lung Cancer Therapy and Antibacterial Applications.

ACS omega·2026
Same author

Heterointerface-Induced Charge Transfer Kinetics in Sputtered MoTe<sub>2</sub>-MoS<sub>2</sub> Nanocomposite for High-Performance Ambient NO<sub>2</sub> Detection.

ACS applied materials & interfaces·2026
Same author

Epidemiological investigation of Chandipura virus disease outbreak, Gujarat, India, July 2024: a matched case-control study.

BMC infectious diseases·2026
Same author

A deep learning multi-attention Bi-GRU framework for k<sub>cat</sub> prediction with segmentation-based insights.

Enzyme and microbial technology·2026
Same author

Sex Differences in Bed Nucleus of the Stria Terminalis Response to Calcitonin Gene-Related Peptide.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Jun 29, 2026

The Preparation of Oblique Spinal Cord Slices for Ventral Root Stimulation
09:10

The Preparation of Oblique Spinal Cord Slices for Ventral Root Stimulation

Published on: October 13, 2016

10.1K

Nicotinic Modulation of Fast-Spiking Neurons in Rat Somatosensory Cortex across Development.

Catherine W Haga1, Jeffrey Koenig2, Nathan Cramer2

  • 1Department of Neurobiology and UM-MIND, University of Maryland School of Medicine, Baltimore, Maryland 21201 chaga@som.umaryland.edu.

Eneuro
|November 17, 2025
PubMed
Summary

Nicotine impacts fast-spiking (FS) neurons during development by increasing synaptic input frequency. However, FS neurons show no direct response to nicotine, possibly due to the expression of lynx1, a nicotinic modulator.

More Related Videos

Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp
10:44

Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp

Published on: June 20, 2018

10.3K
Author Spotlight: Deciphering Neural Circuit Formation from Two-Photon Microscopy and Single Neuron Imaging
06:18

Author Spotlight: Deciphering Neural Circuit Formation from Two-Photon Microscopy and Single Neuron Imaging

Published on: November 21, 2023

1.3K

Related Experiment Videos

Last Updated: Jun 29, 2026

The Preparation of Oblique Spinal Cord Slices for Ventral Root Stimulation
09:10

The Preparation of Oblique Spinal Cord Slices for Ventral Root Stimulation

Published on: October 13, 2016

10.1K
Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp
10:44

Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp

Published on: June 20, 2018

10.3K
Author Spotlight: Deciphering Neural Circuit Formation from Two-Photon Microscopy and Single Neuron Imaging
06:18

Author Spotlight: Deciphering Neural Circuit Formation from Two-Photon Microscopy and Single Neuron Imaging

Published on: November 21, 2023

1.3K

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Neuropharmacology

Background:

  • Signaling via nicotinic acetylcholine receptors (nAChRs) is crucial for developing cerebral cortical circuits.
  • Fast-spiking (FS) inhibitory cortical neurons play a key role in shaping these circuits.
  • Dysfunction in FS neuron nicotinic signaling is linked to neurodevelopmental and psychiatric disorders.

Purpose of the Study:

  • To establish a developmental timeline of pre- and postsynaptic responses to nicotine in FS neurons.
  • To investigate the reasons behind the lack of postsynaptic nicotine response in FS neurons despite receptor expression.

Main Methods:

  • Electrophysiological recordings in primary somatosensory cortex of male and female rats.
  • Analysis of spontaneous synaptic inputs.
  • Quantitative assessment of nicotinic acetylcholine receptor (nAChR) subunit and lynx1 mRNA expression.

Main Results:

  • Nicotine increased spontaneous synaptic input frequency to FS neurons during the second postnatal week, an effect that persisted through development.
  • FS neurons exhibited no postsynaptic responses to nicotine from early identification.
  • FS neurons expressed abundant nAChR subunit mRNA and lynx1 mRNA, with lynx1 expression increasing significantly during the second postnatal week.

Conclusions:

  • FS neurons receive functional presynaptic nicotinic modulation during development.
  • The lack of postsynaptic response is not due to receptor desensitization but may be explained by the presence and upregulation of lynx1, a negative nicotinic modulator.
  • These findings provide insights into the role of nicotinic signaling in cortical circuit development and its potential implications for neurological disorders.