Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

4.1K
Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
4.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Compact Behavioral Model Quantifying the Relationship between Optoelectronic Memristor Dynamics and Reservoir Computing Performance.

The journal of physical chemistry letters·2026
Same author

Study on HSA and Dex-40 Mitigating Osmotic Damage in NK Cells During CPA Loading.

Macromolecular bioscience·2026
Same author

Programmable Cooling-Free Cryopreservation via Multi-Parameter Optimization of Natural Deep Eutectic Solvents.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

Enhanced Preservation of HEK293T Cells and Rat Kidneys by Optimizing Double-Phase Isochoric Freezing with Active Nucleation Management.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

Genome-wide analysis of the CCT gene family in Liriodendron chinense highlights potential functions in light signaling and chlorophyll biosynthesis.

BMC plant biology·2025
Same author

A PRF-Based Multibaseline fitting model for temperature prediction.

Magnetic resonance imaging·2025

Related Experiment Video

Updated: Jan 11, 2026

Preparation of Binary and Ternary Deep Eutectic Systems
06:15

Preparation of Binary and Ternary Deep Eutectic Systems

Published on: October 31, 2019

12.7K

Natural Deep Eutectic Solvents Enable Programmable Cooling-Free Cryopreservation.

Hengxin Han1,2,3, Ying Wang1,2,3, Taijie Zhan1,2,3

  • 1Institute of Biothermal Science and Technology, University of Shanghai for Science and Technology, Shanghai 200093, China.

Langmuir : the ACS Journal of Surfaces and Colloids
|November 18, 2025
PubMed
Summary

Natural deep eutectic solvents (NADES) like PS21 and PS31 show promise as cryoprotectants, significantly improving cell viability and reducing ice damage compared to DMSO for biobanking applications.

More Related Videos

Cryogenic Sample Loading into a Magic Angle Spinning Nuclear Magnetic Resonance Spectrometer that Preserves Cellular Viability
06:42

Cryogenic Sample Loading into a Magic Angle Spinning Nuclear Magnetic Resonance Spectrometer that Preserves Cellular Viability

Published on: September 1, 2020

3.8K
Modified MicroSecure Vitrification: A Safe, Simple and Highly Effective Cryopreservation Procedure for Human Blastocysts
09:35

Modified MicroSecure Vitrification: A Safe, Simple and Highly Effective Cryopreservation Procedure for Human Blastocysts

Published on: March 2, 2017

12.4K

Related Experiment Videos

Last Updated: Jan 11, 2026

Preparation of Binary and Ternary Deep Eutectic Systems
06:15

Preparation of Binary and Ternary Deep Eutectic Systems

Published on: October 31, 2019

12.7K
Cryogenic Sample Loading into a Magic Angle Spinning Nuclear Magnetic Resonance Spectrometer that Preserves Cellular Viability
06:42

Cryogenic Sample Loading into a Magic Angle Spinning Nuclear Magnetic Resonance Spectrometer that Preserves Cellular Viability

Published on: September 1, 2020

3.8K
Modified MicroSecure Vitrification: A Safe, Simple and Highly Effective Cryopreservation Procedure for Human Blastocysts
09:35

Modified MicroSecure Vitrification: A Safe, Simple and Highly Effective Cryopreservation Procedure for Human Blastocysts

Published on: March 2, 2017

12.4K

Area of Science:

  • Cryobiology
  • Biomaterials Science
  • Sustainable Chemistry

Background:

  • Cryopreservation is crucial for long-term storage of biological samples.
  • Conventional cryoprotective agents, such as dimethyl sulfoxide (DMSO), can exhibit toxicity.
  • There is a need for effective, low-toxicity cryoprotectants for improved biobanking.

Purpose of the Study:

  • To evaluate natural deep eutectic solvents (NADES), specifically PS21 and PS31, as novel cryoprotectants.
  • To assess the physicochemical properties and cryoprotective efficacy of these NADES.
  • To compare the performance of NADES with traditional cryoprotectants like DMSO.

Main Methods:

  • Physicochemical characterization using FTIR, DSC, and cryomicroscopy.
  • Biological assays evaluating cell viability (A549 cells) and post-thaw recovery.
  • Optimization of cooling protocols to determine optimal cryopreservation parameters.

Main Results:

  • PS21 and PS31 significantly inhibited ice crystallization and reduced recrystallization damage.
  • NADES demonstrated higher unfrozen water content compared to DMSO.
  • Cell viability and proliferation rates were significantly higher with NADES compared to DMSO, especially with optimized cooling protocols.

Conclusions:

  • NADES (PS21 and PS31) are effective, low-toxicity cryoprotectants.
  • These solvents offer a sustainable and scalable alternative for biobanking.
  • NADES enhance sample quality and streamline cryopreservation workflows.