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Related Experiment Video

Updated: Jan 11, 2026

Production of Nanofibrillar Patterned Collagen for Tissue Engineering
07:34

Production of Nanofibrillar Patterned Collagen for Tissue Engineering

Published on: September 20, 2024

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Catalyst-Free Collagen Filament Crosslinking for Engineering Anisotropic and Mechanically Robust Tissue Scaffolds.

JuYeon Kim1, Hanjun Hwangbo1, ByungJoon Choi1

  • 1Department of Precision Medicine, Sungkyunkwan University School of Medicine (SKKU-SOM), Suwon, 16419, Republic of Korea.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|November 18, 2025
PubMed
Summary
This summary is machine-generated.

Researchers developed reinforced collagen hydrogels using a novel bioorthogonal crosslinking method. These mechanically strong, aligned scaffolds support stem cell growth and promote functional muscle regeneration in vivo.

Keywords:
anisotropic engineered scaffoldsbioorthogonal crosslinkingcollagen hydrogelwet‐spinning

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Developing mechanically resilient hydrogels from natural proteins like collagen is crucial for tissue regeneration.
  • Simultaneously achieving cell compatibility and structural integrity in these hydrogels presents a significant challenge.

Purpose of the Study:

  • To engineer mechanically reinforced, aligned collagen hydrogels using a bioorthogonal crosslinking strategy.
  • To evaluate the cytocompatibility and efficacy of these scaffolds in promoting functional muscle regeneration.

Main Methods:

  • Fabrication of dense collagen hydrogels via bioorthogonal crosslinking with rhodamine and polyethylene glycol (PEG).
  • Integration of crosslinking with wet-spinning to produce uniaxially aligned collagen filaments.
  • Encapsulation of human adipose-derived stem cells (hASCs) within aligned filaments and assessment of mechanotransductive signaling.

Main Results:

  • The bioorthogonal crosslinking method enhanced hydrogel stiffness and mechanical strength.
  • Aligned collagen filaments supported hASC encapsulation and activated mechanotransductive signaling, including cytoskeletal organization and myogenic gene expression.
  • Cell-laden filaments promoted in vitro differentiation and in vivo functional muscle regeneration in a murine volumetric muscle loss model.

Conclusions:

  • The developed strategy provides a scalable, cytocompatible platform for creating aligned, protein-based scaffolds with tunable properties.
  • This approach advances the toolkit for regenerative medicine, particularly for applications requiring mechanically robust and biologically active tissue constructs.