Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

BAP1 deficiency-induced SKA3 promotes clear cell renal cell carcinoma malignant progression by regulating chromosomal instability.

Cell death & disease·2026
Same author

Multi-omics and developmental comparison of direct and conventional warming methods in vitrified human and mouse cleavage-stage embryos.

Human reproduction (Oxford, England)·2026
Same author

Cuproptosis-Related Genes in Immune Infiltration and Diagnosis in Hepatitis B Virus-Related Acute Liver Failure.

Exploration (Beijing, China)·2026
Same author

A ROS-Responsive DNA Nanodevice for Targeted Cytosolic siRNA Delivery in Metabolic Dysfunction-Associated Steatohepatitis.

Journal of the American Chemical Society·2026
Same author

A dual-bioinspired structural design enables highly efficient microwave absorption and thermal insulation in SiC hybrid aerogels.

Materials horizons·2026
Same author

Quantitative RNA pseudouridine landscape reveals dynamic modification patterns and evolutionary conservation across bacterial species.

eLife·2026

Related Experiment Video

Updated: Jan 11, 2026

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage
10:59

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage

Published on: August 21, 2021

4.0K

Single-Cell Super-Resolution Quantification of Oxidative DNA Damage via Aptamer-Assisted DNA-PAINT.

Jingfang Zhao1, Liu Liu1, Yunpeng Song1

  • 1College of Chemistry and Molecular Sciences, State Key Laboratory of Metabolism and Regulation in Complex Organisms, College of Life Sciences, Taikang Center for Life and Medical Sciences, Wuhan University, Wuhan 430072, China.

Analytical Chemistry
|November 18, 2025
PubMed
Summary

Researchers developed a super-resolution imaging method to precisely quantify oxidative DNA damage (8-oxo-dG) in single cells. This technique offers superior specificity and efficiency for studying DNA repair and drug effects.

More Related Videos

Author Spotlight: Quantitative Assessment of 8-oxo-dG in MCF-7 Cells Using ELISA
05:13

Author Spotlight: Quantitative Assessment of 8-oxo-dG in MCF-7 Cells Using ELISA

Published on: May 24, 2024

1.7K
Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter
12:15

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter

Published on: May 29, 2019

9.1K

Related Experiment Videos

Last Updated: Jan 11, 2026

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage
10:59

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage

Published on: August 21, 2021

4.0K
Author Spotlight: Quantitative Assessment of 8-oxo-dG in MCF-7 Cells Using ELISA
05:13

Author Spotlight: Quantitative Assessment of 8-oxo-dG in MCF-7 Cells Using ELISA

Published on: May 24, 2024

1.7K
Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter
12:15

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter

Published on: May 29, 2019

9.1K

Area of Science:

  • Molecular Biology
  • Biophysics
  • Cellular Imaging

Background:

  • Quantifying oxidative DNA damage at the single-cell level is challenging with conventional methods.
  • Ensemble assays obscure cell-to-cell variability and lack molecular specificity.
  • 8-oxo-dG is a key marker of oxidative stress and DNA damage.

Purpose of the Study:

  • To develop a super-resolution imaging strategy for precise, single-cell quantification of 8-oxo-dG.
  • To overcome limitations of existing methods in specificity and resolution.
  • To enable direct evaluation of DNA repair mechanisms and drug efficacy at the single-cell level.

Main Methods:

  • Developed a super-resolution imaging strategy combining an 8-oxo-dG-specific DNA aptamer with DNA-PAINT.
  • Achieved ~22 nm spatial resolution for visualizing 8-oxo-dG lesions in individual cells.
  • Utilized the method to assess the repair efficiency of hOGG1 and its mutants.

Main Results:

  • Successfully visualized and quantified 8-oxo-dG lesions with high spatial resolution.
  • Demonstrated superior specificity and labeling efficiency compared to antibody-based methods.
  • Enabled direct, single-cell evaluation of DNA repair efficiency, independent of transfection variability.

Conclusions:

  • The developed DNA-PAINT aptamer strategy provides a powerful tool for single-cell oxidative DNA damage quantification.
  • This method facilitates accurate assessment of DNA repair dynamics and the impact of therapeutic agents.
  • The platform is adaptable for mapping other small molecular targets in nucleic acids with single-cell precision.