Related Experiment Video For Multiple myeloma
Updated: Jan 11, 2026
Interphase Fluorescence in situ Hybridization of Bone Marrow Smears of Multiple Myeloma
Published on: April 15, 2022
The Relationship between Cytogenetic Abnormalities and Clinical Biological Characteristics in Newly Diagnosed
1Department of Laboratory Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
Objective:
The chromosomal abnormality (CA) in newly diagnosed multiple myeloma (NDMM) suggests the involvement in disease initiation and progression. Fluorescence in situ hybridization (FISH) provides high-sensitivity detection of these aberrations. To elucidate the clinicopathological impact of CAs, we integrated bone marrow (BM) morphology, FISH, flow cytometry (FCM), and PET/CT to analyze their interrelationships in a cohort of 156 NDMM patients.
Methods:
BM morphology, FISH, FCM, and PET/CT were performed in 156 patients with NDMM. MM-related serum and urinary markers were collected.
Results:
Gain or amplification of 1q (gain/amp(1q)), del(13q), del(17p), and immunoglobulin heavy chain (IGH) translocation were observed in 43.59%, 31.41%, 7.69%, and 25% of NDMM patients, respectively. 16 NDMM patients had extramedullary disease (EMD). The SUVmax median was 5.05 (3.93-7.4). Median BM plasma cell ratio and abnormal plasma cell ratio in NDMM patients were 22.25% and 6.475%, respectively. The above cell ratios were inversely correlated with IgA and IgM, positively correlated with creatinine, serum, and urinary β-2 microglobulin, and were proved lower in NDMM patients without CAs. In IgG type NDMM, M protein was significantly associated with the two ratios. Abnormal plasma cell ratio was lower in CD56 negative group and BM plasma cell ratio was lower in R-ISS I stage group.
Conclusions:
The NDMM patients with CAs had higher BM plasma cell ratio, serum and urinary β-2 microglobulin, 24-hour urinary protein, and abnormal plasma cell ratio than the NDMM patients without CAs. The application of BM morphology, FISH, and FCM has significance for the prognosis of NDMM.
