The regulatory MaIAA1-like-MaERF003 module modulates the ripening of "Fenjiao" bananas
Zunyang Song1,2, Hangcong Chen1, Yujie Zhao1
1Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Engineering Research Center for Postharvest Technology of Horticultural Crops in South China, Ministry of Education, College of Horticulture, South China Agricultural University, Guangzhou, Guangdong 510642, China.
Abstract:
Fruit ripening is a complex process mediated by plant hormones and transcriptional regulation. Ethylene and auxin are key regulators, but little is known about their crosstalk in fruit ripening. This study discovered the synergistic effect of auxin and ethylene during "Fenjiao" banana (Musa ABB Pisang Awak) fruit ripening, which is regulated by transcription factors MaIAA1-like and MaERF003. Both ethylene and auxin enhanced ripening and the expression of MaIAA1-like and MaERF003. However, 1-methylcyclopropene (1-MCP) treatment suppressed ripening and the expression of both genes. MaIAA1-like and MaERF003 activated the transcripts of genes associated with chlorophyll, starch, and cell wall degradation. Transient overexpression of MaIAA1-like and MaERF003 in "Fenjiao" banana enhanced ripening by accelerating softening and de-greening and by stimulating the expression of genes related to chlorophyll, starch, and cell wall degradation, while the transient silencing of MaIAA1-like and MaERF003 delayed softening and de-greening. Ectopic overexpression of MaIAA1-like and MaERF003 in tomato (Solanum lycopersicum) also accelerated fruit ripening. MaIAA1-like regulated MaERF003 transcription by directly interacting with its promoter. Additionally, the exogenous application of IAA mitigated the effect of 1-MCP on ripening in "Fenjiao" banana fruit, alleviating the ripening disorder induced by unsuitable 1-MCP. Our findings show that the MaIAA1-like-MaERF003 regulator module participates in the auxin-ethylene signal crosstalk to mediate ripening in "Fenjiao" banana.
More Related Videos
06:11Author Spotlight: Improved Methods for Preparing Transverse Sections and Unrolled Whole Mounts of Maize Leaf Primordia for Fluorescence and Confocal Imaging
Published on: September 22, 2023
10:29Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput
Published on: March 30, 2018
Related Concept Videos
Fruit Development, Structure, and Function
Feedback Regulation of Calcium Concentration
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
Regulation of Transpiration by Stomata
Cell Signaling in Plants
Gene Regulation During Sporulation
Riboswitches
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
