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Updated: Jan 11, 2026

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
Published on: November 16, 2019
Compact way to generate guide star for wavefront sensing in Bessel two-photon microscopy
Fanglin Luo1,2, Nan Li1,2, Chengliang Yang1,2
1State Key Laboratory of Advanced Manufacturing for Optical Systems, Chinese Academy of Sciences, Changchun, 130033, China.
Abstract:
We introduce a compact method for generating guide stars to enable direct wavefront sensing in Bessel two-photon microscopy. By sequentially modulating an axicon phase and a thin lens phase using a spatial light modulator (SLM), our approach achieves seamless switching between Bessel and Gaussian illumination beams without requiring mechanical movement or additional optical components. The Bessel beam provides an expanded field of view for imaging, while the Gaussian beam serves as a point-like guide star for wavefront sensing. Experimental validation in a Bessel two-photon light sheet microscope demonstrates that wavefront correction based on our method enhances the signal-to-noise ratio (SNR) by 7.3 times and improves axial resolution by 4.4 times.
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