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Related Concept Videos

CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR and crRNAs02:53

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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Homologous Recombination02:31

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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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Updated: Jan 11, 2026

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
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Genome Editing in Mammalian Cell Lines using CRISPR-Cas

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Harnessing artificial intelligence to advance CRISPR-based genome editing technologies.

Tyler Thomson1,2, Gen Li1,2, Amy Strilchuk2

  • 1Institute of Biomedical Engineering, University of Toronto, Toronto, Ontario, Canada.

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|November 18, 2025
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Artificial intelligence (AI) accelerates genome editing technologies like CRISPR by optimizing gene editors and discovering new enzymes. AI-powered virtual cell models offer further potential for guiding gene editing strategies and predicting outcomes.

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Area of Science:

  • Genomics
  • Bioengineering
  • Computational Biology

Background:

  • CRISPR technologies revolutionized genome editing for research and medicine.
  • Genetic diseases present significant therapeutic challenges.
  • Artificial intelligence (AI) is emerging as a powerful tool in biological sciences.

Purpose of the Study:

  • To review AI methodologies applied to genome editing.
  • To discuss AI's role in optimizing and discovering genome-editing tools.
  • To explore future opportunities for AI in gene editing.

Main Methods:

  • Review of AI methodologies (machine learning, deep learning).
  • Analysis of AI applications in CRISPR-based genome editing.
  • Discussion of AI-powered virtual cell models.

Main Results:

  • AI accelerates optimization of gene editors for various targets.
  • AI aids in engineering existing genome-editing tools.
  • AI supports the discovery of novel genome-editing enzymes.

Conclusions:

  • AI integration is crucial for advancing genome editing technologies.
  • AI-powered virtual cells can guide target selection and outcome prediction.
  • AI promises significant future impact on genome editing research and therapeutics.