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Measurement of Extracellular Ion Fluxes Using the Ion-selective Self-referencing Microelectrode Technique
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SISE, free LabView-based software for ion flux measurements.

Namrah Ahmad1, Krishani Tennakoon1, Rainer Hedrich2,3

  • 1Molecular Plant Physiology and Biophysics, Julius-von-Sachs Institute for Biosciences, Biocenter, Würzburg University, Julius-von-Sachs-Platz 2, D-97082, Würzburg, Germany.

Plant Methods
|November 19, 2025
PubMed
Summary
This summary is machine-generated.

Researchers developed new software tools, SISE-Monitor and SISE-Analyser, to improve the measurement and analysis of plant ion fluxes using Scanning Ion-Selective Electrodes (SISE). These free tools enhance the study of mineral uptake and plant development.

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Area of Science:

  • Plant Physiology
  • Biophysics
  • Analytical Chemistry

Background:

  • Plant growth relies on mineral nutrient uptake and transport.
  • Electrophysiological techniques are crucial for studying ion transport and signaling in plants.
  • Scanning Ion-Selective Electrodes (SISE) offer a non-invasive method for measuring ion fluxes.

Purpose of the Study:

  • To develop user-friendly software for Scanning Ion-Selective Electrodes (SISE) measurements.
  • To facilitate the recording and analysis of ion flux data in plants.
  • To provide freely accessible tools for researchers studying plant ion transport.

Main Methods:

  • Development of two LabView-based software programs: SISE-Monitor for data acquisition and SISE-Analyser for data analysis.
  • Implementation of automated electrode movement and data processing routines.
  • Provision of executable files for routine use and source code for customization.

Main Results:

  • Successful creation of SISE-Monitor and SISE-Analyser software.
  • Enabled efficient and accurate recording and analysis of plant ion fluxes.
  • Made software freely available with source code for further development.

Conclusions:

  • The developed LabView software enhances the application of SISE for studying plant ion transport.
  • Freely available software lowers barriers for researchers investigating mineral uptake and plant development.
  • The open-source nature allows for integration with other measurement techniques, such as fluorescent dye imaging.