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Related Concept Videos

Formation of Lipopolysaccharides01:19

Formation of Lipopolysaccharides

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Lipopolysaccharides (LPS) are crucial components of the outer membrane of Gram-negative bacteria, serving both structural and functional roles. It contributes to membrane stability and protects bacteria from host immune responses. LPS is composed of three major regions—lipid A, a core oligosaccharide, and an O antigen. The biosynthesis and assembly of LPS involve a highly coordinated set of enzymatic reactions and transport mechanisms. Additionally, LPS is recognized as an endotoxin,...
515

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LPS-Binding Hydrogel for TLR4-Mediated Microbiota-Immune Modulation.

Jiali Chen1, Chenzhou Wu1, Renjie Yang2

  • 1State Key Laboratory of Oral Diseases and National Clinical Research Center for Oral Diseases and Department of Oral and Maxillofacial Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan, 610041, China.

Advanced Materials (Deerfield Beach, Fla.)
|November 19, 2025
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Summary
This summary is machine-generated.

A novel hydrogel effectively clears lipopolysaccharide (LPS) from wounds, restoring bacterial balance and promoting tissue healing. This biomaterial combines antimicrobial and immune-modulating properties for enhanced regenerative therapy.

Keywords:
hydrogellipopolysaccharidemicrobiotaoronasal‐perforating woundpolymyxin B

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Area of Science:

  • Biomaterials Science
  • Immunology
  • Regenerative Medicine

Background:

  • Lipopolysaccharide (LPS) from Gram-negative bacteria activates the immune system and hinders tissue repair during microbial imbalance.
  • Current LPS-scavenging methods struggle to access lipid A, the core component, due to shielding by bacterial structures.
  • Oronasal wounds, common in cleft palate repair, are susceptible to infection and healing complications.

Purpose of the Study:

  • To develop a synergistic hydrogel (OCMC-PMBP) for effective LPS capture and bacterial lysis.
  • To investigate the role of LPS-Toll-like receptor 4 (TLR4) signaling in immune dysregulation and impaired healing of oronasal wounds.
  • To evaluate the hydrogel's efficacy in resolving dysbiosis-induced inflammation and promoting regenerative healing.

Main Methods:

  • Development of a dual-action hydrogel combining polymyxin B (PMB) and polyethyleneimine (PEI).
  • Application of the hydrogel to oronasal-perforating wound models in mice.
  • Analysis of the wound microbiome, immune cell phenotypes, and tissue regeneration using 16S rRNA sequencing, metagenomics, and single-cell transcriptomics.

Main Results:

  • The OCMC-PMBP hydrogel significantly reduced LPS levels and restored microbial balance.
  • Treatment suppressed inflammation and accelerated epithelial regeneration and collagen remodeling in wound sites.
  • The hydrogel modulated immune cell phenotypes and cell-cell interactions, promoting a pro-regenerative environment.

Conclusions:

  • The OCMC-PMBP hydrogel offers a promising strategy for managing LPS-driven inflammation and enhancing healing in complex mucosal wounds.
  • This biomaterial design integrates antimicrobial and immunomodulatory functions to address dysbiosis and promote tissue regeneration.
  • Targeting LPS and restoring immune homeostasis are crucial for effective wound healing in infection-prone conditions.