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Integrative Transcriptomic and Phosphoproteomic Analysis Reveals Key Components of the SnRK1 Signaling Network in

Maria C Faria-Bates1, Chandan Maurya1, K Muhammed Jamsheer2,3

  • 1Department of Crop, Soil, and Environmental Sciences University of Arkansas System Division of Agriculture Fayetteville Arkansas USA.

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|November 19, 2025
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Summary
This summary is machine-generated.

The SNF1/AMPK-related kinase 1 (SnRK1) in rice regulates growth based on energy status. This study reveals SnRK1’s dual role in promoting growth and mediating stress responses, uncovering new signaling pathways.

Keywords:
SnRK1–TOR signalingphosphoproteomicsprotein phosphorylationproteomicsstarvationtranscriptomics

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Area of Science:

  • Plant molecular biology
  • Biochemistry
  • Genetics

Background:

  • SnRK1 (SNF1/AMPK-related kinase 1) is a crucial energy sensor regulating growth in plants.
  • Understanding SnRK1 signaling in monocots, like rice, is limited despite its known adaptive and developmental roles.

Purpose of the Study:

  • To comprehensively analyze the SnRK1 signaling network in rice using CRISPR/Cas9 mutagenesis.
  • To elucidate SnRK1's function in growth and stress adaptation under varying energy conditions.

Main Methods:

  • CRISPR/Cas9 gene editing to create rice *snrk1* mutants.
  • Phenotypic, transcriptomic, proteomic, and phosphoproteomic analyses were performed.

Main Results:

  • *snrk1* mutants exhibited growth defects under normal and starvation conditions.
  • SnRK1 signaling influences both growth and stress-related processes, with evidence of kinase subunit subfunctionalization.
  • Novel SnRK1 targets in membrane trafficking, ethylene signaling, and ion transport were identified.

Conclusions:

  • SnRK1 acts as a growth promoter under favorable conditions and a critical regulator of adaptive stress responses.
  • The study deepens the understanding of SnRK1's complex roles in plant physiology and adaptation.