Comprehensive Bioinformatic Analysis Reveals Survival-Associated Hub Genes and MicroRNAs in Multiple Myeloma Patients

Area of Science:

• Oncology
• Molecular Biology
• Genomics

Background:

• Multiple myeloma (MM) is a B-cell malignancy with limited curative options.
• Fundamental molecular insights are needed for improved MM treatment.

Purpose of the Study:

• Identify key genes and microRNAs (miRNAs) in multiple myeloma (MM).
• Analyze their roles in disease progression, prognosis, and therapeutic resistance.

Main Methods:

• Re-analyzed transcriptomic datasets (GSE16558, GSE141260, GSE146649) using high-throughput sequencing.
• Identified differentially expressed genes (DEGs) and miRNAs (DEMs).
• Performed Gene Ontology (GO), pathway enrichment, and protein-protein interaction (PPI) network analyses.

Main Results:

• Identified 13 common DEGs enriched in immune cell migration, lymphocyte activation, and TGF-β signaling.
• CCND1, ITGB1, and CREB1 identified as hub genes significantly associated with survival.
• miR-34c-5p and miR-155-5p implicated in apoptosis and drug resistance.

Conclusions:

• Complex interplay between genetic alterations and immune microenvironment in MM.
• Potential for novel biomarkers and therapeutic targets for MM management.
• Improved strategies for treatment, prognosis, and overcoming therapeutic resistance.