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CircFNIP1 Regulates Proliferation and Invasion in Lung Cancer Cells and Represents a Potential Therapeutic Target
Dan Lan1,2, Yingjun Liu1, Yan Zhang1
1Department of Oncology, The First Affiliated Hospital of Jinan University, Guangzhou, China.
Iranian Journal of Biotechnology
|November 19, 2025
Summary
Circular RNA FNIP1 (circFNIP1) promotes lung cancer growth and metastasis. Targeting circFNIP1 with antisense oligonucleotides suppressed tumor growth, indicating its potential as an RNA-based therapeutic strategy for lung cancer.
Area of Science:
- Oncology
- Molecular Biology
- RNA Therapeutics
Background:
- Lung cancer (LC) is a leading cause of cancer mortality.
- Circular RNAs (circRNAs) play crucial roles in cancer development.
- circFNIP1 (hsa_circ_0073858) is investigated for its role in LC.
Purpose of the Study:
- To investigate the functional role of circFNIP1 in lung cancer.
- To evaluate the therapeutic potential of targeting circFNIP1 in LC.
- To identify circFNIP1-associated molecular mechanisms in LC.
Main Methods:
- Quantitative real-time PCR and fluorescence in situ hybridization for circFNIP1 expression and localization.
- siRNA-mediated knockdown of circFNIP1 in LC cell lines (PC-9, SPC-A1).
- In vitro assays (CCK-8, colony formation, flow cytometry, wound healing, transwell) and in vivo xenograft models in nude mice.
- Bioinformatics analyses to predict miRNA and downstream targets.
Main Results:
- circFNIP1 is highly expressed in LC cells, localized in the nucleus and cytoplasm.
- circFNIP1 knockdown significantly inhibited proliferation, colony formation, migration, and invasion in vitro.
- circFNIP1 knockdown suppressed tumor growth in vivo; bioinformatics identified miR-1231/miR-657 and ICAM1 as key targets.
Conclusions:
- circFNIP1 acts as an oncogenic circRNA in lung cancer.
- circFNIP1 is a promising target for RNA-based therapeutic strategies in LC.
- Findings support the development of circFNIP1-targeted interventions for LC management.
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