Janus Hydrogel Microparticles with Tea Polyphenol/Melanin Nanoparticles Integration from Microfluidics for Wound...

Area of Science:

• Biomaterials Science
• Nanotechnology
• Wound Healing Research

Background:

• Bioactive compounds show promise for wound healing but face challenges in combined application due to mutual interference.
• Developing effective strategies for synergistic delivery of multiple therapeutic agents is crucial for advanced wound care.

Purpose of the Study:

• To develop Janus hydrogel microparticles co-loaded with tea polyphenol-magnesium and melanin nanoparticles for synergistic antioxidant and antibacterial wound treatment.
• To evaluate the efficacy of these Janus microparticles in promoting diabetic wound healing.

Main Methods:

• Preparation of tea polyphenol-magnesium nanoparticles via self-assembly.
• Harvesting melanin nanoparticles from black hair.
• Co-loading nanoparticles into Janus hydrogel microparticles using microfluidics technology.
• In vitro biocompatibility, antioxidant, and antibacterial activity assessments.
• In vivo evaluation of diabetic wound healing promotion.

Main Results:

• The Janus microparticles demonstrated high biocompatibility.
• Enhanced antioxidant performance and synergistic antibacterial activity were observed.
• Application to diabetic wounds promoted healing by scavenging reactive oxygen species, killing bacteria, reducing inflammation, decreasing matrix metalloproteinase-9 secretion, and enhancing tissue remodeling.

Conclusions:

• Janus hydrogel microparticles co-loaded with tea polyphenol-magnesium and melanin nanoparticles represent a promising strategy for synergistic wound treatment.
• These microparticles show significant potential for clinical application in enhancing wound healing, particularly in complex cases like diabetic wounds.