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  1. Home
  3. Ridge-assisted Micro Positioning Of Cells And Particles In A Microchannel.
  1. Home
  3. Ridge-assisted Micro Positioning Of Cells And Particles In A Microchannel.

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Ridge-assisted Micro Positioning of Cells and Particles in a Microchannel.

Adriana Payan-Medina1,2,3,4, Victor R Putaturo2,3,4, Sajad R Bazaz2,3,4

  • 1Harvard-MIT Division of Health Sciences and Technology, Cambridge, Massachusetts 02139, USA.

Biorxiv : the Preprint Server for Biology
|November 19, 2025

View abstract on PubMed

Summary
This summary is machine-generated.

We developed a new microfluidic technique called ridge-assisted micro positioning (RAMP) for precise cell stream control. RAMP enables deterministic lateral focusing of cells, independent of size or flow rate, for biomedical applications.

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Area of Science:

  • Microfluidics
  • Biomedical Engineering
  • Cellular Biology

Background:

  • Precise lateral control of cell streams in microchannels is crucial for applications like flow cytometry and liquid biopsies.
  • Existing inertial microfluidic methods offer high-throughput cell ordering but lack precise lateral position control.

Purpose of the Study:

  • To present a novel microfluidic method for deterministic lateral positioning of focused cell streams.
  • To demonstrate a technique independent of particle size and flow rate for versatile cell manipulation.

Main Methods:

  • Leveraging engineered microvortices generated by channel ridges and fluidic lift forces.
  • Utilizing ridge-assisted micro positioning (RAMP) to control cell stream lateral positions.
  • Testing RAMP with polydisperse particles (10-30 μm) across a broad flow rate range (250-1000 μL/min).

Main Results:

  • RAMP achieved deterministic lateral focusing of cell streams, independent of particle size and flow rate.
  • Precise adjustment of focusing position was possible by altering ridge placement and geometry, allowing shifts of 5 μm to ≥15 μm.
  • Developed clog-free microfluidic cell concentrators using RAMP, capable of enriching cells in undiluted whole blood.

Conclusions:

  • RAMP offers precise and deterministic control over cell stream lateral positions in microchannels.
  • The technique is versatile, applicable to polydisperse particles and complex biological fluids like whole blood.
  • RAMP establishes a robust platform for advanced cell positioning in various biomedical applications.