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Related Experiment Video

Updated: Jan 11, 2026

Real-Time Void Spot Assay
06:39

Real-Time Void Spot Assay

Published on: February 10, 2023

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Spoti-find: A novel, open-source void spot assay image analysis tool.

Cara C Hardy, Susan Sheehan, Seamus Mawe

    Biorxiv : the Preprint Server for Biology
    |November 19, 2025
    PubMed
    Summary

    Spoti-find is a new, open-source software for analyzing rodent void spot assays (VSA). It offers improved metrics and a user-friendly interface, enhancing the study of urinary dysfunction in preclinical research.

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    Area of Science:

    • Urology
    • Animal Behavior
    • Software Development

    Background:

    • The void spot assay (VSA) is crucial for studying rodent urinary behavior.
    • Existing VSA analysis tools lack comprehensive features, throughput, or accessibility.

    Purpose of the Study:

    • To develop Spoti-find, an open-source VSA image analysis application.
    • To introduce novel metrics and a user-friendly interface for VSA analysis.

    Main Methods:

    • Developed Spoti-find, a stand-alone application with a graphical user interface.
    • Implemented manual/semi-automated identification, adjustable thresholds, and data export.
    • Introduced novel metrics: void circularity, distance to edge, and volume-based void binning.

    Main Results:

    • Spoti-find demonstrated strong inter-user consistency and sensitivity to known phenotypes.
    • The software accurately captured novel parameters with high agreement with existing tools.
    • Validated across diverse datasets, including aging and disease models.

    Conclusions:

    • Spoti-find enhances the interpretive power of VSA by analyzing voiding patterns' behavioral context and spatial morphology.
    • Provides a flexible, user-friendly platform for phenotyping urinary dysfunction in preclinical studies.
    • Expands VSA capabilities for research into aging and disease models.