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Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...

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Related Experiment Video

Updated: May 3, 2026

Multiplex Cytokine Profiling of Stimulated Mouse Splenocytes Using a Cytometric Bead-based Immunoassay Platform

Multiplex Cytokine Profiling of Stimulated Mouse Splenocytes Using a Cytometric Bead-based Immunoassay Platform

Published on: November 9, 2017

IBT-16plex-Based Quantitative Proteomics at the Single-Cell Level: Enabling Protein Profiles toward the Single Cells

Xiaolian Ning^1,2, Jun Li^2,3, Yuying Huang^2,4

¹BGI Research, Shenzhen 518083, China.

Analytical Chemistry

|November 19, 2025

Summary

The novel 16plex isobaric tags (IBT-16plex) reagent shows promise for single-cell proteomics. This study optimized IBT-16plex labeling and demonstrated its comparability to TMTpro-16plex for profiling mouse spleen cells.

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Dynamic Proteomic and miRNA Analysis of Polysomes from Isolated Mouse Heart After Langendorff Perfusion

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Last Updated: May 3, 2026

Multiplex Cytokine Profiling of Stimulated Mouse Splenocytes Using a Cytometric Bead-based Immunoassay Platform

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Published on: November 9, 2017

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

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Dynamic Proteomic and miRNA Analysis of Polysomes from Isolated Mouse Heart After Langendorff Perfusion

Dynamic Proteomic and miRNA Analysis of Polysomes from Isolated Mouse Heart After Langendorff Perfusion

Published on: August 29, 2018

Area of Science:

Proteomics
Mass Spectrometry
Cell Biology

Background:

Isobaric tag-based mass spectrometry is established for single-cell proteomics.
16plex isobaric tags (IBT-16plex) are a novel reagent not yet applied to single-cell analysis.

Purpose of the Study:

To evaluate the suitability of IBT-16plex for proteomic quantification at the single-cell level.
To optimize labeling conditions and assess performance compared to existing reagents.

Main Methods:

Systematic evaluation of IBT-16plex reaction conditions, including mass ratios and carrier protein strategies.
Parallel labeling of single-cell peptides from mouse spleen tissue using IBT-16plex and TMTpro-16plex.
Bioinformatic analysis for protein identification, quantification, and cell type clustering.

Main Results:

Optimal IBT-16plex labeling achieved with a mass ratio >10,000 and a 200-fold excess of carrier proteins.
IBT-16plex relative quantification demonstrated comparability to TMTpro-16plex.
Successful profiling and quantification of single cells from mouse spleen, clustering into monocytes, dendritic cells, macrophages, and T cells.

Conclusions:

IBT-16plex is a viable and effective reagent for single-cell proteomics.
This method enables proteomic analysis of single cells isolated directly from animal tissues.