Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Chemotaxis and Direction of Cell Migration01:21

Chemotaxis and Direction of Cell Migration

4.3K
Cells can detect chemical cues in their environment and reorganize the cytoskeleton to migrate toward them or away from them. This directional migration, called chemotaxis, is essential during embryogenesis and development, immune response, tissue repair and regeneration, and reproduction. These chemical cues can either attract or repel the cell's movement. For example, axon development is determined by a combination of chemoattractants and chemorepellents that direct the growing axon...
4.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effective SU(2) gadget: holonomic walk on higher-order Poincaré sphere.

Optics express·2026
Same author

Transfer Learning in Bone Scintigraphy: Superior to Early-career Readers and Comparable to Experts in Classifying Skeletal Metastasis.

Nuclear medicine and molecular imaging·2025
Same author

Early detection and a treatment bundle strategy for postpartum haemorrhage: a mixed-methods process evaluation.

The Lancet. Global health·2025
Same author

Advanced AI and ML frameworks for transforming drug discovery and optimization: With innovative insights in polypharmacology, drug repurposing, combination therapy and nanomedicine.

European journal of medicinal chemistry·2024
Same author

SISCOS in focal cortical dysplasia: localization and comparative analysis with MRI.

Neuroradiology·2024
Same author

Innovative approaches to Alzheimer's therapy: Harnessing the power of heterocycles, oxidative stress management, and nanomaterial drug delivery system.

Ageing research reviews·2024

Related Experiment Video

Updated: Jan 11, 2026

An All-on-chip Method for Rapid Neutrophil Chemotaxis Analysis Directly from a Drop of Blood
07:21

An All-on-chip Method for Rapid Neutrophil Chemotaxis Analysis Directly from a Drop of Blood

Published on: June 23, 2017

17.5K

A Novel Image-Based Approach for Analyzing Neutrophil Chemotaxis Using a Boyden Chamber Assay.

Mohammad Umar1, Gurpreet Kaur Aulakh1

  • 1Department of Small Animal Clinical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.

Current Protocols
|November 19, 2025
PubMed
Summary

A new image analysis method enhances neutrophil chemotaxis assays by quantifying all cell migration stages. This provides a more comprehensive understanding of immune cell behavior in inflammation research.

Keywords:
Boyden chamberFiji/ImageJchemotaxismembrane transmigration indexneutrophils

More Related Videos

Microfluidic Platform for Measuring Neutrophil Chemotaxis from Unprocessed Whole Blood
10:13

Microfluidic Platform for Measuring Neutrophil Chemotaxis from Unprocessed Whole Blood

Published on: June 3, 2014

11.7K
Imaging G Protein-coupled Receptor-mediated Chemotaxis and its Signaling Events in Neutrophil-like HL60 Cells
08:24

Imaging G Protein-coupled Receptor-mediated Chemotaxis and its Signaling Events in Neutrophil-like HL60 Cells

Published on: September 14, 2016

10.5K

Related Experiment Videos

Last Updated: Jan 11, 2026

An All-on-chip Method for Rapid Neutrophil Chemotaxis Analysis Directly from a Drop of Blood
07:21

An All-on-chip Method for Rapid Neutrophil Chemotaxis Analysis Directly from a Drop of Blood

Published on: June 23, 2017

17.5K
Microfluidic Platform for Measuring Neutrophil Chemotaxis from Unprocessed Whole Blood
10:13

Microfluidic Platform for Measuring Neutrophil Chemotaxis from Unprocessed Whole Blood

Published on: June 3, 2014

11.7K
Imaging G Protein-coupled Receptor-mediated Chemotaxis and its Signaling Events in Neutrophil-like HL60 Cells
08:24

Imaging G Protein-coupled Receptor-mediated Chemotaxis and its Signaling Events in Neutrophil-like HL60 Cells

Published on: September 14, 2016

10.5K

Area of Science:

  • Immunology and Inflammation Research
  • Cell Biology
  • Microscopy and Image Analysis

Background:

  • Chemotaxis is crucial for immune responses like inflammation, wound healing, and defense.
  • Neutrophils are key immune cells that migrate to infection/injury sites.
  • Current neutrophil chemotaxis assays have limitations in capturing all cell migration stages.

Purpose of the Study:

  • To develop an improved image analysis method for neutrophil chemotaxis.
  • To provide a more comprehensive and physiologically relevant understanding of neutrophil migration dynamics.
  • To offer a more sensitive and accurate tool for immune cell behavior research.

Main Methods:

  • Development of a novel image analysis technique for the Boyden chamber.
  • Quantification of neutrophils in three stages: adherent, transmigrating, and transmigrated.
  • Application in analyzing bone marrow-derived neutrophil migration.

Main Results:

  • The new method accounts for all neutrophil migration stages, unlike traditional methods.
  • Provides a more complete picture of neutrophil chemotaxis.
  • Enhances sensitivity and accuracy in dissecting immune cell dynamics.

Conclusions:

  • The developed image analysis method offers a significant advancement in studying neutrophil chemotaxis.
  • Enables deeper insights into immune responses and inflammatory diseases.
  • Facilitates the development of potential therapeutic interventions.