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Updated: Jun 20, 2026
13:13
High Efficiency Differentiation of Human Pluripotent Stem Cells to Cardiomyocytes and Characterization by Flow Cytometry
Published on: September 23, 2014
30.8K
Dynamic proteome profiling of differentiating human embryonic stem cells towards cardiomyocytes
Anna Meyfour1, Sara Pahlavan2, Samaneh Maleknia3
1Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran. a.meyfour@sbmu.ac.ir.
Scientific Reports
|November 19, 2025
Summary
This study reveals novel molecular mechanisms in heart development by profiling proteomes during cardiomyocyte differentiation. Key processes like ferroptosis and sirtuin signaling were identified at specific developmental stages.
Area of Science:
- Developmental Biology
- Proteomics
- Cardiovascular Research
Background:
- Heart development is crucial but incompletely understood.
- Previous proteomic studies focused on late-stage cardiomyogenesis.
Purpose of the Study:
- To comprehensively profile the proteome across key in vitro cardiomyocyte differentiation stages.
- To identify novel molecular mechanisms and biomarkers for heart development.
Main Methods:
- Quantitative proteomic analysis using multiplexed tandem mass tag-based mass spectrometry.
- Profiling of mesendoderm, cardiac mesoderm, cardiac progenitor cell, and cardiomyocyte stages.
- Unsupervised clustering and pathway enrichment analysis.
Main Results:
- 4417 differentially expressed proteins were identified, clustering into six key biological processes.
- Transitional-specific proteins were most abundant in the cardiac mesoderm stage.
- Ferroptosis and sirtuin signaling pathways were implicated in specific differentiation stages.
Conclusions:
- Stepwise proteome analysis provides novel insights into cardiomyocyte differentiation.
- Identified proteins expand the potential biomarker repertoire for developmental stages.
- Uncovered novel molecular mechanisms underlying early heart development.
Keywords:
Cardiac developmentCardiac progenitor cellsCardiomyocyteFerroptosisProteomicsSirtuin signalingMore Related Videos
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