A highly sensitive and regenerable electrochemical immunosensor chip for blood adiponectin detection

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Clinical Diagnostics

Background:

• Type 2 Diabetes Mellitus (T2DM) poses a significant global health challenge.
• Early detection and sensitive diagnosis are crucial for managing T2DM.
• Adiponectin is a validated biomarker for T2DM, showing high accuracy in early stages.

Purpose of the Study:

• To develop a novel and reusable electrochemical immunosensor for adiponectin detection.
• To facilitate early warning and intervention strategies for T2DM.
• To create a sensitive and reliable diagnostic tool for clinical application.

Main Methods:

• Immobilization of adiponectin antibodies on a working electrode modified with gold nanoparticles (AuNPs).
• Fabrication of an electrochemical immunosensor chip.
• Integration of the sensing unit into a flow cell for continuous operation.

Main Results:

• The immunosensor demonstrated high sensitivity (3.03 μA/decade) and a wide linear range (0.5-25,000 ng/mL).
• A low limit of detection (LOD) of 0.0093 ng/mL was achieved.
• The sensor exhibited excellent repeatability, reproducibility, and anti-interference capabilities over 14 regeneration cycles.

Conclusions:

• The developed electrochemical immunosensor is a promising tool for sensitive and early detection of T2DM via adiponectin measurement.
• The sensor's reusability and performance in real blood samples support its potential for clinical diagnosis.
• This technology can aid in timely intervention and management of Type 2 Diabetes Mellitus.