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A Multi-Omics Dataset of Prostate Cancer Response to Oncolytic Virus OH2 Treatment
Jinzhou Xu1, Guichen Ye1, Ye An1
1Department and Institute of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Abstract:
Prostate cancer is the most common malignant tumor of the male genitourinary system, especially metastatic castration resistant prostate cancer (mCRPC), which currently lacks effective treatment methods. Immunotherapy has limited efficacy in prostate cancer, partly due to its immunosuppressive "cold" tumor microenvironment (TME). Oncolytic virus therapy, such as OH2 modified based on herpes simplex virus type 2, provides a new strategy for reshaping TME and enhancing immune activation. However, the complex regulatory mechanism of oncolytic viruses on TME has not been fully elucidated. This study used multi omics integrated analysis (transcriptomics, metabolomics, and single-cell RNA sequencing) to investigate the effect of OH2 intervention on prostate cancer TME. We systematically analyzed the gene expression changes, metabolic reprogramming, and immune cell dynamics after OH2 treatment using in vitro cell models and RM-1 mouse subcutaneous transplant tumor models. The dataset includes gene expression profiles, metabolic profiles, and single-cell transcriptomes, providing a resource for investigating OH2-induced changes in the prostate cancer TME.
Insights
Oncolytic virus OH2 therapy may reshape the immunosuppressive tumor microenvironment (TME) in prostate cancer. This study investigates OH2's effects on TME using multi-omics, offering insights into novel cancer treatments.
Area of Science:
- Oncology
- Immunology
- Virology
- Genomics
- Metabolomics
Background:
- Metastatic castration-resistant prostate cancer (mCRPC) presents significant therapeutic challenges.
- Current immunotherapies show limited efficacy in prostate cancer due to the "cold" tumor microenvironment (TME).
- Oncolytic viruses offer a promising strategy to modify the TME and enhance anti-tumor immunity.
Purpose of the Study:
- To elucidate the complex regulatory mechanisms of oncolytic viruses on the prostate cancer TME.
- To investigate the effects of OH2, a modified herpes simplex virus type 2, on the prostate cancer TME.
- To provide a comprehensive resource for understanding OH2-induced TME alterations.
Main Methods:
- Utilized multi-omics integrated analysis, including transcriptomics, metabolomics, and single-cell RNA sequencing.
- Employed in vitro cell models and RM-1 mouse subcutaneous transplant tumor models for investigation.
- Systematically analyzed gene expression, metabolic reprogramming, and immune cell dynamics post-OH2 treatment.
Main Results:
- Detailed analysis of gene expression profiles revealed significant alterations in the TME following OH2 intervention.
- Metabolomic profiling identified key metabolic reprogramming events induced by OH2 treatment.
- Single-cell RNA sequencing elucidated dynamic changes in immune cell populations and their functions within the TME.
Conclusions:
- OH2 therapy demonstrates potential in modulating the prostate cancer TME, shifting it towards a more immune-permissive state.
- The multi-omics approach provides a deep understanding of OH2's mechanisms of action in prostate cancer.
- This study lays the groundwork for developing more effective oncolytic virus-based immunotherapies for mCRPC.
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