Related Experiment Video
Updated: Jan 10, 2026

The Use of Fluorescent Target Arrays for Assessment of T Cell Responses In vivo
Published on: June 19, 2014
Engineering tandem VHHs to target different epitopes to enhance antibody-dependent cell-mediated cytotoxicity
Yuqiang Xu1, Hao Jiang1, Limin Chen1
1Research & Development Department, Tavotek Biotherapeutics, Suzhou, China.
Abstract:
Engineering antibodies to elicit antibody-dependent cell-mediated cytotoxicity (ADCC) can be used to eliminate target cells. Here, we described how single domain VHH arms on a bispecific antibody (BsAb) format can be engineered to modulate cell binding and ADCC activity. The BsAbs, comprising two anti-epidermal growth factor receptor (EGFR) nanobodies, 7D12 and EGA1, were engineered onto a human IgG1 Fc domain in monovalent, bivalent, and tandem formats. While 7D12 had a stronger ADCC activity than EGA1, the tandem 7D12-EGA1 mediated a significantly stronger ADCC activity without significant changes in cell binding in numerous cancer cell lines. In addition, we present how the molecular design of the tandem 7D12 and EGA1 nanobodies could cross-link two different EGFR molecules to obtain stronger ADCC activity.
More Related Videos
12:42Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo
Published on: January 7, 2019
08:10Simultaneous Quantification of Anti-vector and Anti-transgene-Specific CD8+ T Cells Via MHC I Tetramer Staining After Vaccination with a Viral Vector
Published on: November 28, 2018
Related Concept Videos
Tumor Immunotherapy
Hybridoma Technology
Hybridoma Selection
Commonly used fusion techniques — electroporation,...
Cytotoxic T Cells-mediated Immune Response
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...