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Updated: Jul 2, 2026
Generation of Murine Monoclonal Antibodies by Hybridoma Technology
Published on: January 2, 2017
Generation of Rat Monoclonal Antibodies for Human and Mouse Androgen Receptor
Momo Koike1, Tomoka Kawabata1, Norikazu Kiguchi2
1Department of Chemistry and Bioengineering, Graduate School of Engineering, Osaka Metropolitan University, Osaka, Japan.
Abstract:
Androgen receptor (AR) is activated by binding to androgens, which leads to nuclear translocation, dimerization, and binding to androgen response elements (AREs) to regulate gene transcription. AR is important in masculinization during mammalian development and is a major driver of tumor growth in prostate cancer, for which AR pathway inhibitors are the standard treatment. However, the mechanisms by which AR participates in these processes remain unclear. In this study, we describe rat monoclonal antibodies (mAbs) that were generated against human and mouse AR. These mAbs recognize endogenous AR and were shown to be effective in the immunofluorescence staining of human cell lines and mouse tissue sections and in immunoprecipitation experiments. We expect these mAbs to be useful for functional analyses of AR.
Insights
Researchers developed rat monoclonal antibodies (mAbs) targeting the androgen receptor (AR). These antibodies effectively detect endogenous AR in cells and tissues, aiding future functional studies of AR in development and prostate cancer.
Area of Science:
- Molecular Biology
- Immunology
- Cell Biology
Background:
- The androgen receptor (AR) is crucial for mammalian development and prostate cancer progression.
- Understanding AR's mechanisms is vital, as AR pathway inhibitors are standard prostate cancer treatments.
- Current knowledge of AR's precise roles in these processes remains incomplete.
Purpose of the Study:
- To generate novel rat monoclonal antibodies (mAbs) against human and mouse androgen receptor (AR).
- To validate the utility of these mAbs for detecting endogenous AR in various biological contexts.
- To provide tools for advancing functional analyses of AR.
Main Methods:
- Generation of rat monoclonal antibodies against human and mouse AR.
- Immunofluorescence staining of human cell lines and mouse tissue sections.
- Immunoprecipitation experiments to confirm AR binding.
Main Results:
- Successfully generated rat monoclonal antibodies recognizing endogenous AR.
- Demonstrated antibody effectiveness in immunofluorescence and immunoprecipitation assays.
- Confirmed cross-reactivity with both human and mouse AR.
Conclusions:
- The developed mAbs are valuable tools for studying AR.
- These antibodies enable visualization and detection of AR in cellular and tissue samples.
- The mAbs are expected to facilitate further research into AR's functions in development and disease.
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