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Updated: Jan 10, 2026

Growth-based Determination and Biochemical Confirmation of Genetic Requirements for Protein Degradation in Saccharomyces cerevisiae
Published on: February 16, 2015
Co-expression of a retrotransposon and re-targeted restriction factor impairs yeast growth
1Department of Biology, Hope College, Holland, Michigan, United States.
Abstract:
Retrotransposons and retroviruses shape genome evolution and can negatively impact genome function. Saccharomyces cerevisiae minimizes replication of Ty1 family retrotransposons using an inhibitory protein identical to the C-terminal domain of the transposon's capsid protein. We previously elucidated the molecular basis of restriction and subfamily specific targeting and engineered a re-targeted restriction factor. Here, we report that co-expression of the Ty1' retrotransposon and a variant of its restriction factor mutated to re-target a related transposon subfamily impairs yeast growth.
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