A set of serum proteomic biomarkers differentiates celiac children from age and human leukocyte antigen-matched healthy controls
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View abstract on PubMed
Summary
This summary is machine-generated.Researchers identified seven serum biomarkers that can differentiate celiac disease (CD) patients from healthy individuals with over 90% accuracy. This discovery could lead to a noninvasive "liquid biopsy" for diagnosing CD, reducing the need for intestinal biopsies.
Area Of Science
- Gastroenterology and Immunology
- Biomarker Discovery
- Molecular Diagnostics
Background
- Celiac disease (CD) diagnosis often requires invasive small intestinal biopsies.
- New European Society of Gastroenterology Hepatology and Nutrition (ESPGHAN) criteria aim to reduce biopsy necessity, but a noninvasive diagnostic method is still needed.
- Serum biomarkers offer potential for a
- liquid biopsy
- approach to CD diagnosis.
Purpose Of The Study
- To identify a panel of serum biomarkers capable of distinguishing children with CD from age- and human leukocyte antigen (HLA)-matched healthy controls.
- To explore the potential of these biomarkers for a noninvasive CD diagnostic strategy.
Main Methods
- Utilized proximity extension immunoassay technology (Olink Proteomics®) with the ProSeek Multiplex Inflammation panel.
- Analyzed the relative concentrations of 92 inflammation-linked proteins in the sera of 50 children with CD and 50 healthy controls.
- Employed multivariate statistical analyses including univariate and multivariate distribution analysis, random forest classification, and linear discriminant analysis.
Main Results
- Identified a cluster of seven specific molecules (CASP8, CXCL9, NT-3, SIRT2, STAMBP, ST1A1, and TNFSF14) with significant diagnostic potential.
- These seven biomarkers demonstrated the ability to differentiate CD patients from controls with approximately 90% accuracy (95% CI; 0.7-0.99).
Conclusions
- Celiac disease patients exhibit elevated serum levels of specific inflammatory molecules compared to controls.
- The identified seven-protein signature accurately differentiates CD cases from controls, suggesting a role in NF-κB cytokine signaling, apoptosis, and crypt proliferation.
- This panel of biomarkers holds promise for developing a noninvasive, individualized approach to celiac disease diagnosis in clinical settings.
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