Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

19.9K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
19.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Rust fungi (Pucciniales, Basidiomycota) of the Brazilian Cerrado: Taxonomic advances and new taxa in a threatened biome.

Mycologia·2026
Same author

Resistance to Triazoles in Populations of <i>Mycosphaerella fijiensis</i> and <i>M. musicola</i> from the Sigatoka Disease Complex from Commercial Banana Plantations in Minas Gerais and São Paulo, Brazil.

Microorganisms·2025
Same author

<i>Pratylenchus brasiliensis</i> sp. nov. (Nematoda: Pratylenchidae): An Emerging Threat to Soybean in an Important New Agricultural Frontier in Brazil.

Plant disease·2025
Same author

Microscopic Characterization of the Infectious Process, ROS Production, and Fungi Cellular Death of Alternaria alternata on Tangerine Resistant to QoIs.

Microscopy research and technique·2024
Same author

Target and non-target site mechanisms of fungicide resistance and their implications for the management of crop pathogens.

Pest management science·2023
Same author

Higher fitness and competitive advantage of Pyricularia oryzae Triticum lineage resistant to QoI fungicides.

Pest management science·2022

Related Experiment Video

Updated: Jan 10, 2026

Measuring Interactions between Fluorescent Probes and Lignin in Plant Sections by sFLIM Based on Native Autofluorescence
07:15

Measuring Interactions between Fluorescent Probes and Lignin in Plant Sections by sFLIM Based on Native Autofluorescence

Published on: January 2, 2020

5.9K

Method for Lignin Analysis in Wood by Fluorescence Microscopy.

Josy Tainara Silva Silva1, Silvino Intra Moreira2, Jordão Cabral Moulin1

  • 1Department of Forestry and Wood Sciences, Federal University of Espírito Santo-UFES, Jerônimo Monteiro, Brazil.

Microscopy Research and Technique
|November 20, 2025
PubMed
Summary

This study validated fluorescence microscopy for analyzing lignin in Amazonian woods. Autofluorescence, especially when automated, proved effective for quantifying lignin content at the cellular level.

Keywords:
ImageJ softwareMäuleautofluorescencebasic fuchsin

More Related Videos

Label-free in situ Imaging of Lignification in Plant Cell Walls
07:35

Label-free in situ Imaging of Lignification in Plant Cell Walls

Published on: November 1, 2010

12.8K
Ultrafast Lignin Extraction from Unusual Mediterranean Lignocellulosic Residues
09:22

Ultrafast Lignin Extraction from Unusual Mediterranean Lignocellulosic Residues

Published on: March 9, 2021

7.4K

Related Experiment Videos

Last Updated: Jan 10, 2026

Measuring Interactions between Fluorescent Probes and Lignin in Plant Sections by sFLIM Based on Native Autofluorescence
07:15

Measuring Interactions between Fluorescent Probes and Lignin in Plant Sections by sFLIM Based on Native Autofluorescence

Published on: January 2, 2020

5.9K
Label-free in situ Imaging of Lignification in Plant Cell Walls
07:35

Label-free in situ Imaging of Lignification in Plant Cell Walls

Published on: November 1, 2010

12.8K
Ultrafast Lignin Extraction from Unusual Mediterranean Lignocellulosic Residues
09:22

Ultrafast Lignin Extraction from Unusual Mediterranean Lignocellulosic Residues

Published on: March 9, 2021

7.4K

Area of Science:

  • Wood Anatomy
  • Biochemistry
  • Microscopy

Background:

  • Native Amazonian species exhibit significant anatomical variation impacting lignin content.
  • Accurate lignin quantification is crucial for understanding wood properties and applications.

Purpose of the Study:

  • To evaluate different fluorescence treatments and image processing methods for lignin analysis in native Amazonian woods.
  • To establish a reliable fluorescence-based method for quantifying lignin content at the cellular level.

Main Methods:

  • Seven native Amazonian wood species were analyzed using the Klason method for total lignin content.
  • Histological sections underwent four fluorescence treatments: autofluorescence (in natura, without extractives), basic fuchsin, and Mäule.
  • Fluorescence intensity was measured using ImageJ with three processing methodologies (two density formulas, one automated).

Main Results:

  • Autofluorescence without extractives, analyzed automatically (Method 3), showed the strongest correlation (R²=76.22%, r=0.87) between fluorescence intensity and lignin content.
  • Automated analysis ensured speed, reproducibility, and standardization of results.
  • The study validated fluorescence intensity as a reliable indicator of lignin content in histological sections.

Conclusions:

  • Fluorescence microscopy, particularly automated autofluorescence analysis, is a reliable and efficient method for studying lignin at the cellular level in native Amazonian woods.
  • This technique offers a safe and standardized approach for lignin quantification, complementing traditional methods.