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Solution stability and storage effect on selected metallodrugs: a multi-technique evaluation
Luca Famlonga1, Riccardo Di Leo2, Lorenzo Chiaverini1
1Department of Pharmacy, University of Pisa, Via Bonanno Pisano 6, 56126 Pisa, Italy.
Metallomics : Integrated Biometal Science
|November 20, 2025
Summary
Assessing the solution stability of inorganic drugs is crucial for accurate biological testing. Systematic stability studies using multiple techniques prevent misleading results and ensure reliable metallodrug development.
Area of Science:
- Inorganic chemistry
- Medicinal chemistry
- Pharmaceutical sciences
Background:
- Inorganic drugs are vital in medicine, but their behavior in solution is often overlooked.
- Speciation, hydrolysis, and redox reactions significantly impact drug efficacy, safety, and reproducibility in biological tests.
Purpose of the Study:
- To demonstrate the necessity of systematic stability assessment for inorganic drugs before biological evaluation.
- To establish a framework for integrating solution stability studies into metallodrug development.
Main Methods:
- Selected four diverse metallodrugs: ruthenium(III) complex NAMI-A, platinum(II) drug oxaliplatin, platinum(IV) derivative Hex-Pt, and gold(I) complex Npx-Au.
- Employed an integrated, multi-technique approach combining NMR spectroscopy, UV-Vis spectroscopy, and HPLC-MS.
Main Results:
- Reliably detected early degradation events in metallodrugs.
- Defined optimal storage conditions for maintaining drug integrity.
- Identified and avoided potential sources of misleading experimental outcomes.
Conclusions:
- Rigorous stability profiling is essential for accurate dosing, reproducibility, and correct interpretation of preclinical assays.
- Systematic solution stability evaluation ensures more reliable translation of metallodrugs from bench to clinic.
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