Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Extraction: Advanced Methods00:56

Extraction: Advanced Methods

1.1K
Metal ions can be separated from one another by complexation with organic ligands–the chelating agent– to form uncharged chelates. Here, the chelating agent must contain hydrophobic groups and behave as a weak acid, losing a proton to bind with the metal. Since most organic ligands used in this process are insoluble or undergo oxidation in the aqueous phase, the chelating agent is initially added to the organic phase and extracted into the aqueous phase. The metal-ligand complex is...
1.1K
Effects of EDTA on End-Point Detection Methods01:18

Effects of EDTA on End-Point Detection Methods

602
Different methods, such as visual observance of metal-ion indicators, spectroscopic techniques, and potentiometric methods, can determine the endpoint of an EDTA titration.
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a...
602
Complexation Equilibria: Factors Influencing Stability of Complexes01:09

Complexation Equilibria: Factors Influencing Stability of Complexes

774
In complexation reactions, metal cations are the electron pair acceptors, and the ligands are the electron pair donors. The stability of the metal complexes depends primarily on the complexing ability of the central metal ion and the nature of the ligands. Generally, the complexing ability of the metal ion depends on the size and charge of the ion. As the metal ion size increases, the stability of the metal complexes decreases, provided that the valency of the metal ion and the ligands remain...
774
Drug Product Stability01:16

Drug Product Stability

218
The long-term stability of drug products is critical to ensuring their quality, safety, and effectiveness over time. Stability directly influences a product's ability to maintain its intended characteristics, ensuring it performs as expected during its intended shelf life. Key attributes such as drug potency, impurities, dissolution, and other physicochemical measures of performance are tested to assess stability. These parameters indicate how well the product retains its quality over time and...
218
Complexometric Titration: Ligands00:43

Complexometric Titration: Ligands

2.2K
Different monodentate and polydentate ligands are used as complexing agents in complexometric titration reactions. The formation of complexes by mono- and bidentate ligands involves two or more intermediate steps, limiting their use as complexing agents. In comparison, polydentate ligands can form complexes with metal ions in a single-step process, facilitating sharper end points. This means polydentate ligands, such as amino carboxylic acid derivatives, are most commonly employed in...
2.2K
Therapeutic Drug Monitoring: Drug Analysis Methods01:26

Therapeutic Drug Monitoring: Drug Analysis Methods

166
Therapeutic Drug Monitoring (TDM) is a clinical practice that measures specific drug levels in a patient's blood or body tissues to tailor drug therapy effectively. This monitoring is critical for managing drugs with narrow therapeutic indices like digoxin and phenytoin, ensuring they are both safe and effective. For instance, monitoring theophylline levels in asthma patients involves precision and sensitivity to adjust doses according to individual responses to therapy, ensuring efficacy and...
166

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Dual-Action Gold(I) Prodrug Targeting Redox Homeostasis and Extracellular Matrix Remodeling in Ovarian Cancer.

ACS medicinal chemistry letters·2026
Same author

Targeting Copper in Cancer: Chelators to Counteract Cuproplasia and Ionophores to Promote Cuproptosis.

Journal of medicinal chemistry·2026
Same author

Tuning Au Reactivity Beyond Canonical Targets: Ligand-Driven Au(I) Metalation of Lysine Residues in Hen Egg White Lysozyme.

Inorganic chemistry·2026
Same author

Functional PET imaging of gut microbiota with [<sup>18</sup>F]fluorodeoxyglucose, [<sup>18</sup>F]fluorodeoxysorbitol and [<sup>11</sup>C]choline reflects Clostridia and Lactobacillales abundance in caecum and small intestine and host metabolic interactions.

European journal of nuclear medicine and molecular imaging·2026
Same author

Dual actionability of BMI1 activation and mitotic vulnerability defines adaptive Osimertinib resistance in EGFR-mutant NSCLC.

Cell death & disease·2026
Same author

A brain-penetrant chimeric protein combines direct glioma targeting with myeloid cell-mediated immune reprogramming.

Journal of translational medicine·2026

Related Experiment Video

Updated: Jan 10, 2026

Using Multiple Light Scattering to Examine the Stability of Phyllanthus emblica L. Extracts Obtained with Different Extraction Methods
06:12

Using Multiple Light Scattering to Examine the Stability of Phyllanthus emblica L. Extracts Obtained with Different Extraction Methods

Published on: April 14, 2023

947

Solution stability and storage effect on selected metallodrugs: a multi-technique evaluation.

Luca Famlonga1, Riccardo Di Leo2, Lorenzo Chiaverini1

  • 1Department of Pharmacy, University of Pisa, Via Bonanno Pisano 6, 56126 Pisa, Italy.

Metallomics : Integrated Biometal Science
|November 20, 2025
PubMed
Summary

Assessing the solution stability of inorganic drugs is crucial for accurate biological testing. Systematic stability studies using multiple techniques prevent misleading results and ensure reliable metallodrug development.

More Related Videos

Clean Sampling and Analysis of River and Estuarine Waters for Trace Metal Studies
10:44

Clean Sampling and Analysis of River and Estuarine Waters for Trace Metal Studies

Published on: July 1, 2016

11.9K
Methods of Ex Situ and In Situ Investigations of Structural Transformations: The Case of Crystallization of Metallic Glasses
08:55

Methods of Ex Situ and In Situ Investigations of Structural Transformations: The Case of Crystallization of Metallic Glasses

Published on: June 7, 2018

8.9K

Related Experiment Videos

Last Updated: Jan 10, 2026

Using Multiple Light Scattering to Examine the Stability of Phyllanthus emblica L. Extracts Obtained with Different Extraction Methods
06:12

Using Multiple Light Scattering to Examine the Stability of Phyllanthus emblica L. Extracts Obtained with Different Extraction Methods

Published on: April 14, 2023

947
Clean Sampling and Analysis of River and Estuarine Waters for Trace Metal Studies
10:44

Clean Sampling and Analysis of River and Estuarine Waters for Trace Metal Studies

Published on: July 1, 2016

11.9K
Methods of Ex Situ and In Situ Investigations of Structural Transformations: The Case of Crystallization of Metallic Glasses
08:55

Methods of Ex Situ and In Situ Investigations of Structural Transformations: The Case of Crystallization of Metallic Glasses

Published on: June 7, 2018

8.9K

Area of Science:

  • Inorganic chemistry
  • Medicinal chemistry
  • Pharmaceutical sciences

Background:

  • Inorganic drugs are vital in medicine, but their behavior in solution is often overlooked.
  • Speciation, hydrolysis, and redox reactions significantly impact drug efficacy, safety, and reproducibility in biological tests.

Purpose of the Study:

  • To demonstrate the necessity of systematic stability assessment for inorganic drugs before biological evaluation.
  • To establish a framework for integrating solution stability studies into metallodrug development.

Main Methods:

  • Selected four diverse metallodrugs: ruthenium(III) complex NAMI-A, platinum(II) drug oxaliplatin, platinum(IV) derivative Hex-Pt, and gold(I) complex Npx-Au.
  • Employed an integrated, multi-technique approach combining NMR spectroscopy, UV-Vis spectroscopy, and HPLC-MS.

Main Results:

  • Reliably detected early degradation events in metallodrugs.
  • Defined optimal storage conditions for maintaining drug integrity.
  • Identified and avoided potential sources of misleading experimental outcomes.

Conclusions:

  • Rigorous stability profiling is essential for accurate dosing, reproducibility, and correct interpretation of preclinical assays.
  • Systematic solution stability evaluation ensures more reliable translation of metallodrugs from bench to clinic.