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Using Low-Speed Rotation in Heparin Immobilization Improved Antithrombogenicity of Tubular Acellular Vascular
Hoang Minh Lam1,2,3, Nho Thuan Nguyen1,2,3, My Thi Ngoc Nguyen1,2,3
1Laboratory of Tissue Engineering and Biomedical Materials, University of Science, 227 Nguyen Van Cu Street, Cho Quan Ward, Ho Chi Minh City, Vietnam.
Tissue Engineering and Regenerative Medicine
|November 20, 2025
Summary
This study improved acellular vascular grafts by using a roller-assisted Layer-by-Layer (LbL) method to immobilize heparin. The enhanced scaffolds show reduced thrombus formation and improved hemocompatibility for better vascular regeneration.
Area of Science:
- Biomaterials Science
- Vascular Tissue Engineering
- Surface Chemistry
Background:
- Acellular vascular scaffolds lack anticoagulant properties, increasing thrombus risk.
- Thrombogenicity hinders clinical use of small-diameter vascular grafts.
Purpose of the Study:
- To enhance the hemocompatibility of acellular vascular scaffolds.
- To investigate heparin immobilization using a roller-assisted Layer-by-Layer (LbL) technique.
Main Methods:
- Heparin immobilization on decellularized scaffolds via electrostatic interactions using a DHI-based linker.
- Utilized a roller tube system for low-speed rotation LbL assembly.
- Optimized heparin loading through 1, 4, 7, 10, 13 deposition cycles for sustained release.
Main Results:
- Achieved sustained heparin release over 28 days with prolonged anticoagulant activity.
- Significantly reduced thrombus formation and exhibited minimal hemolytic activity.
- Promoted endothelial cell adhesion, crucial for vascular integrity and patency.
Conclusions:
- Roller-assisted LbL heparinization is effective for improving blood compatibility of vascular grafts.
- This scalable strategy addresses thrombogenicity in vascular tissue engineering.
- The method shows promise for clinical translation of bioengineered vascular constructs.
