Effect of different enzyme pretreatment on the structure and stability of soybean oil body

Herein, the effect of pretreatment with cellulase and hemicellulase under neutral pH conditions on the extraction rate and stability of oil bodies (OB) was investigated. The results showed that cellulase pretreatment led to a higher extraction yield (85.07 %) compared to untreated OB (13.11 %) and hemicellulase-pretreated OB (81.61 %). Protein content and microstructural analysis revealed that cellulase pretreatment resulted in more exogenous proteins on the OB interface, with a more uniform protein distribution. Spectroscopic techniques and SDS-PAGE analysis have confirmed that enzyme pretreatment affects the structure and interactions of interfacial proteins. Cellulase treatment inserted more protein chains into the lipid acyl chains, while increasing the β-sheet content without damaging OB structure. These structural changes improved OB's stability, with cellulase-pretreated OB showing the best large amplitude oscillatory shear (LAOS) properties, environmental and oxidative stability. In conclusion, this study provides a foundation for the development of green, efficient methods for OB extraction.