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Updated: Jan 10, 2026
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Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus
Published on: April 23, 2014
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Flexible oocyte manipulation with delayed maturation and improved somatic cell nuclear transfer efficiency using
Luis H de Aguiar1, Yoke Lee Lee2, Abdallah W Abdelhady2
1Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, 14853, USA; Author's present address: Department of Large Animal Clinical Sciences, University of Florida, Gainesville, FL, 32608, USA.
Theriogenology
|November 20, 2025
Summary
Delayed oocyte maturation provides flexible timing for bovine somatic cell nuclear transfer (SCNT) without affecting competence. Pluripotent stem cell nuclear transfer (PSCNT) using bovine-induced pluripotent stem cells significantly improves embryo development, enhancing cloning efficiency in cattle.
Area of Science:
- Reproductive Biotechnology
- Developmental Biology
- Stem Cell Science
Background:
- Somatic cell nuclear transfer (SCNT) is an important tool in cattle reproduction and research, but its efficiency is limited.
- Improving SCNT efficiency is crucial for advancing cattle breeding and understanding developmental processes.
Purpose of the Study:
- To investigate strategies for enhancing handmade cloning (HMC) efficiency in cattle.
- To evaluate the impact of delayed oocyte maturation on SCNT outcomes.
- To assess the efficacy of using bovine-induced pluripotent stem cells (biPSCs) for pluripotent stem cell nuclear transfer (PSCNT).
Main Methods:
- Bovine oocytes underwent either conventional (20h) or delayed maturation (20h holding + 20h maturation).
- SCNT and PSCNT were performed using fibroblast or biPSC donor cells, respectively.
- Embryo development was assessed by cleavage and blastocyst rates after 7 days of culture.
Main Results:
- Delayed oocyte maturation did not significantly affect oocyte competence for SCNT.
- Pluripotent stem cell nuclear transfer (PSCNT) using biPSCs resulted in significantly higher fusion rates compared to fibroblast SCNT.
- BiPSC-PSCNT significantly enhanced blastocyst development rates compared to fibroblast-SCNT, with improved cleavage and blastocyst formation overall.
Conclusions:
- Delayed oocyte maturation offers a flexible approach to SCNT timing in cattle without compromising developmental potential.
- Pluripotent stem cell nuclear transfer (PSCNT) using biPSCs represents a superior method for improving embryo developmental outcomes in cattle cloning.
- These findings support the advancement of SCNT efficiency and its applications in cattle reproductive biotechnology.
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