Related Experiment Video

Updated: Jan 10, 2026

T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing
08:59

T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing

Published on: January 12, 2021

8.7K

Tissue and peripheral T cell receptor repertoire predicts immunotherapy response and progression-free survival in

Manuel Pino-González1, Martín Lázaro-Quintela1,2, Irene Alonso-Álvarez1

  • 1Translational Oncology Group, Galicia Sur Health Research Institute (IIS Galicia Sur), SERGAS-UVIGO, Hospital Álvaro Cunqueiro, Carretera Clara Campoamor, 341, 36213, Vigo, Spain.

Scientific Reports
|November 20, 2025
PubMed

Insights

T-cell receptor (TCR) repertoire analysis shows promise as a predictive biomarker for immunotherapy response in advanced non-small cell lung cancer (NSCLC) patients treated with pembrolizumab.

Area of Science:

  • Oncology
  • Immunology
  • Genetics

Background:

  • Immunotherapy offers new treatment options for advanced non-small cell lung cancer (NSCLC).
  • Predicting patient response to immunotherapy remains a challenge due to the lack of effective biomarkers.
  • T cells are crucial for anti-tumor immunity, making T cell receptors (TCRs) potential predictive markers.

Purpose of the Study:

  • To evaluate the potential of baseline TCR repertoire as a predictive biomarker for immunotherapy response in advanced NSCLC patients.
  • To assess if TCR repertoire can predict response to first-line pembrolizumab treatment.

Main Methods:

  • Peripheral blood and tissue samples were collected at baseline from advanced NSCLC patients.
  • Next-generation sequencing was performed targeting TCRβ/γ genes.
  • Analysis of tumor-infiltrating and circulating TCR repertoires was conducted.

Main Results:

  • An uneven tumor-infiltrating TCRβ repertoire was observed.
  • Specific tumor-infiltrating and circulating TRBV/J gene usage predicted immunotherapy response.
  • Baseline TCR repertoire analysis demonstrated potential as a predictive biomarker.

Conclusions:

  • Evaluating tissue and circulating TCRβ repertoire before pembrolizumab treatment is supported.
  • TCR repertoire emerges as a promising biomarker for predicting immunotherapy response in NSCLC patients.
Keywords:
Immunotherapy responseNon-small cell lung cancerPembrolizumabPredictive biomarkerT cell receptorTCR repertoire

More Related Videos

Predictive Immune Modeling of Solid Tumors08:50

Predictive Immune Modeling of Solid Tumors

Published on: February 25, 2020

7.4K
In Vitro Tumor Cell Rechallenge For Predictive Evaluation of Chimeric Antigen Receptor T Cell Antitumor Function08:04

In Vitro Tumor Cell Rechallenge For Predictive Evaluation of Chimeric Antigen Receptor T Cell Antitumor Function

Published on: February 27, 2019

12.4K

Related Concept Videos

Tumor Immunotherapy01:27

Tumor Immunotherapy

Immunotherapy is a treatment that boosts or manipulates the immune system to fight diseases, including cancer. For instance, by stimulating an immune response through vaccinations against viruses that cause cancers, like hepatitis B virus and human papillomavirus, these diseases can be prevented. Nonetheless, some cancer cells can avoid the immune system due to their rapid mutation and division. The immune response to many cancers involves three phases: elimination, equilibrium, and escape.
1.7K
Cytotoxic T Cells-mediated Immune Response01:27

Cytotoxic T Cells-mediated Immune Response

Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
6.4K
T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
14.6K