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Amyloid Fibrils03:03

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Amyloid fibrils are aggregates of misfolded proteins.  Under most circumstances, misfolded proteins are either refolded by chaperone proteins or degraded by the proteasome. However, in the case of a mutation or a disease, these proteins can accumulate to form large clusters and often further assemble to form elongated fibers, called fibrils. 
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Emerging Technologies and Integrated Interdisciplinary Strategies for Mitigating Protein Aggregation in Therapeutic

Haomin Wu1, QinXi Fan1, Zheng Zhang2

  • 1Jiangsu Province Hi-Tech Key Laboratory for Biomedical Research, School of Chemistry and Chemical Engineering, Southeast University, No.2 Southeast University Road, Nanjing, 211189, Jiangsu, China.

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Developing therapeutic proteins faces aggregation challenges. New methods in characterization, computational chemistry, and machine learning aid in predicting stability and improving formulation development.

Keywords:
aggregationcharacterizationcomputational chemistrymachine learningtheoretical modeltherapeutic proteins

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Area of Science:

  • Biopharmaceutical development
  • Protein science
  • Drug discovery

Background:

  • Therapeutic proteins are crucial in modern medicine.
  • Protein aggregation remains a significant hurdle in their development.

Purpose of the Study:

  • To review novel research methods for therapeutic proteins.
  • To highlight advancements, limitations, and future directions.

Main Methods:

  • Advanced characterization techniques to identify aggregation.
  • Computational chemistry for microscopic insights into aggregation.
  • Theoretical modeling and machine learning for stability prediction.

Main Results:

  • Methods effectively identify aggregation tendencies and mechanisms.
  • Computational tools offer detailed insights into protein aggregation.
  • Predictive models facilitate high-throughput screening in formulation.

Conclusions:

  • Interdisciplinary collaboration is key for understanding protein aggregation.
  • Integrating diverse approaches drives innovation in protein formulation.
  • Addressing aggregation is vital for advancing therapeutic protein development.