Related Experiment Video
Updated: Jan 10, 2026
06:57
Development of an Antigen-driven Colitis Model to Study Presentation of Antigens by Antigen Presenting Cells to T Cells
Published on: September 18, 2016
9.5K
Mucosal B Cell Expansion and Maturation Contribute to Colitis Pathogenesis
Ivan C N Fung1,2, Pim J Koelink1,2, Lieven G M Mulders3
1Tytgat Institute for Liver and Intestinal Research, Amsterdam UMC, Location University of Amsterdam, Amsterdam, The Netherlands.
Inflammatory Bowel Diseases
|November 21, 2025
Summary
Naive B cells expand in ulcerative colitis (UC) patients and worsen experimental colitis. This suggests a pathogenic role for these specific B cells in active UC, challenging previous therapeutic approaches.
Area of Science:
- Immunology
- Gastroenterology
- Cell Biology
Background:
- Ulcerative colitis (UC) involves chronic colonic inflammation with altered B cell responses.
- Previous attempts to target CD20+ B cells in UC have been ineffective.
Purpose of the Study:
- To investigate the role of naive B cells in ulcerative colitis.
- To explore B cell contributions to colitis severity using a mouse model.
Main Methods:
- Single-cell transcriptomic analysis of colonic biopsies from UC patients and controls.
- Induction of colitis in mice by transferring naive B cells and T cells at varying ratios.
Main Results:
- A distinct subset of naive B cells (MS4A1+CD27-IGHD+TCL1A+) was enriched and more mature in inflamed UC tissues.
- Naive B cells interacted with CD4+ T cell subsets.
- Co-transfer of naive B cells exacerbated colitis in mice, indicated by weight loss and increased inflammation.
Conclusions:
- Naive B cells expand in active UC mucosa.
- Naive B cells play a pathogenic role in experimental colitis, suggesting they are a potential therapeutic target.
Keywords:
B cell maturation and expansionB cell pathologymice colitis transfer modelsingle-cell RNA-sequencingulcerative colitisMore Related Videos
Related Concept Videos
01:27Inflammatory Bowel Disease I: Ulcerative Colitis
876
Introduction
Inflammatory bowel disease, or IBD, encompasses a group of disorders characterized by chronic inflammation or ulceration of the gastrointestinal tract.
Risk Factors
The exact cause of IBD remains unclear, although it is believed to be due to a mix of genetic, environmental, microbial, and immune factors. Genetic factors are significant in determining susceptibility to IBD, with family history being a critical risk factor. Individuals with a first-degree relative who has IBD are at...
Inflammatory bowel disease, or IBD, encompasses a group of disorders characterized by chronic inflammation or ulceration of the gastrointestinal tract.
Risk Factors
The exact cause of IBD remains unclear, although it is believed to be due to a mix of genetic, environmental, microbial, and immune factors. Genetic factors are significant in determining susceptibility to IBD, with family history being a critical risk factor. Individuals with a first-degree relative who has IBD are at...
876
01:23Renewal of Intestinal Stem Cells
3.1K
The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the...
3.1K
01:30Inflammatory Bowel Disease II: Crohn's Disease
932
Introduction
Inflammatory bowel disease, commonly known as IBD, refers to a collection of disorders that lead to persistent inflammation of the gastrointestinal tract. The two types of IBD are ulcerative colitis, which impacts the colon, and Crohn's disease, which can involve any part of the gastrointestinal segment.
Crohn's disease
Crohn's disease is a chronic, systemic inflammatory bowel disease (IBD) that predominantly affects the gastrointestinal tract. It is marked by...
Inflammatory bowel disease, commonly known as IBD, refers to a collection of disorders that lead to persistent inflammation of the gastrointestinal tract. The two types of IBD are ulcerative colitis, which impacts the colon, and Crohn's disease, which can involve any part of the gastrointestinal segment.
Crohn's disease
Crohn's disease is a chronic, systemic inflammatory bowel disease (IBD) that predominantly affects the gastrointestinal tract. It is marked by...
932
01:17Chronic Bowel Disorders: Introduction
735
Chronic bowel diseases are a group of long-term conditions affecting the digestive tract, characterized by inflammation and damage to the gut lining. These conditions primarily include irritable bowel syndrome and inflammatory bowel disease.
Irritable Bowel Syndrome (IBS) is a common disorder affecting the gastrointestinal tract. The distinctive feature is recurrent abdominal pain associated with altered bowel movements, manifesting as constipation, diarrhea, or fluctuating between both. The...
Irritable Bowel Syndrome (IBS) is a common disorder affecting the gastrointestinal tract. The distinctive feature is recurrent abdominal pain associated with altered bowel movements, manifesting as constipation, diarrhea, or fluctuating between both. The...
735
01:24Pathophysiology of Peptic Ulcer Disease: Mucosal Defense Factors
1.1K
Peptic ulcer disease, commonly called PUD, represents a multifaceted condition characterized by disruptions in the lining of the gastrointestinal (GI) tract. Central to the protection of the gastrointestinal lining is the mucosal-bicarbonate barrier. This physiological defense mechanism is a formidable shield against the corrosive effects of gastric acid and pepsin secretion in the stomach. Its role is pivotal in maintaining the structural integrity of the stomach's inner lining.
1.1K
01:24B Cell Activation and Differentiation
15.8K
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
15.8K