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A Novel Interference-Based Supercritical Angle Fluorescence Biosensor.

Finub James Shirley^1,2, Pieter Neutens¹, Ali Amir Saleh^1,3

¹imec, Kapeldreef 75, 3001 Heverlee, Belgium.

|November 21, 2025

Summary

This summary is machine-generated.

This study introduces a novel, miniaturized supercritical angle fluorescence (SAF) biosensor using CMOS technology. The new device achieves higher sensitivity and faster results for protein detection compared to traditional methods.

Keywords:

CMOS biosensor evanescent sensing fluorescence interference filter near-field collection supercritical angle fluorescence

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Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer

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Published on: October 1, 2016

Area of Science:

Biophotonics
Nanotechnology
Biosensing

Background:

Supercritical angle fluorescence (SAF) offers surface-sensitive optical transduction.
Existing SAF biosensors often require complex and costly collection setups.
Total internal reflection fluorescence (TIRF) systems have limitations in excitation optics.

Purpose of the Study:

To develop a simplified and miniaturized SAF transducer using CMOS-compatible technologies.
To enhance SAF biosensor sensitivity and reduce assay time.
To enable cost-effective, scalable manufacturing of SAF devices.

Main Methods:

Designed an interference filter to selectively transmit SAF while blocking excitation light and undercritical angle fluorescence (UAF).
Integrated CMOS-compatible materials for transducer fabrication.
Validated performance using a protein A immunoassay and compared with fluorescence well plates.

Main Results:

Achieved a limit of detection (LoD) of 1 pM for the SAF transducer, outperforming well plates (10 pM).
Demonstrated a washless assay format, significantly reducing time to result.
Utilized CMOS-compatible materials for potential scalable and cost-effective manufacturing.

Conclusions:

The novel interference-based SAF transducer offers a simplified, miniaturized, and highly sensitive platform for biosensing.
CMOS compatibility enables scalable and cost-effective production of advanced optical transducers.
This technology has the potential to revolutionize point-of-care diagnostics and biochemical analysis.