Biomimetic aligned bacterial cellulose dressing containing polydeoxyribonucleotide for promoting wound healing

Area of Science:

• Biomaterials Science
• Regenerative Medicine
• Tissue Engineering

Background:

• Chronic wounds pose significant challenges, driving demand for advanced wound dressings.
• Bacterial cellulose (BC) offers a promising scaffold for wound healing applications.
• Polydeoxyribonucleotide (PDRN) is recognized for its potent tissue-regenerative properties.

Purpose of the Study:

• To develop an aligned bacterial cellulose (BC) membrane loaded with polydeoxyribonucleotide (PDRN) for enhanced wound healing.
• To investigate the physicochemical properties and in vitro/in vivo performance of the PDRN-loaded BC membrane.
• To evaluate the potential of this composite dressing in promoting skin tissue regeneration.

Main Methods:

• Fabrication of an aligned bacterial cellulose membrane.
• Loading of polydeoxyribonucleotide (PDRN) into the aligned BC membrane to create a PDRN@BC aligned membrane.
• Characterization of the composite material's structure, hydrophilicity, air permeability, mechanical properties, and PDRN release kinetics.
• In vitro assessment of L929 fibroblast cell behavior (spreading, proliferation, migration).
• In ovo evaluation of angiogenesis.
• In vivo testing on wound models to assess healing efficacy.

Main Results:

• The PDRN@BC aligned membrane exhibited an aligned structure, high hydrophilicity, good air permeability, and mechanical strength.
• Sustained release of PDRN from the composite membrane was observed.
• The membrane significantly promoted fibroblast cell spreading, proliferation, and migration in vitro.
• Enhanced angiogenesis was observed in ovo.
• In vivo application led to accelerated wound healing, reduced inflammation, and facilitated angiogenesis.

Conclusions:

• The developed PDRN@BC aligned membrane is a promising wound dressing material.
• The combination of topographic cues (aligned structure) and bioactive agents (PDRN) effectively promotes skin tissue regeneration.
• This innovative composite dressing shows significant potential for treating chronic and non-healing wounds.