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Related Concept Videos

Microbial Classification System01:24

Microbial Classification System

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Classification is the process of organizing organisms into hierarchically inclusive groups based on their phenotypic similarities or evolutionary relationships. A species comprises one or more strains, and closely related species are grouped into genera. Genera are further classified into families, families into orders, orders into classes, and so forth, up to the domain level, which is the broadest taxonomic rank derived from a combination of phenotypic and genotypic data.The nomenclature of...
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Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

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Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
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Genomic DNA in Prokaryotes00:46

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The genome of most prokaryotic organisms consists of double-stranded DNA organized into one circular chromosome in a region of cytoplasm called the nucleoid. The chromosome is tightly wound, or supercoiled, for efficient storage. Prokaryotes also contain other circular pieces of DNA called plasmids. These plasmids are smaller than the chromosome and often carry genes that confer adaptive functions, such as antibiotic resistance.
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Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes02:16

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The present-day mitochondrial and chloroplast genomes have retained some of the characteristics of their ancestral prokaryotes and also have acquired new attributes during their evolution within eukaryotic cells. Like prokaryotic genomes, mitochondrial and chloroplast genomes neither bind with histone-like proteins nor show complex packaging into chromosome-like structures, as observed in eukaryotes. Unlike mitotic cell divisions observed in eukaryotic cells, mitochondria and chloroplasts...
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Genome Annotation and Assembly03:36

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The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
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Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale  studies have provided new insights into the evolutionary relationship between organisms.
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Related Experiment Video

Updated: Jan 10, 2026

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
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GlobDB: a comprehensive species-dereplicated microbial genome resource.

Daan R Speth1, Nick Pullen1, Samuel T N Aroney2

  • 1Centre for Microbiology and Environmental Systems Science, University of Vienna, 1030 Vienna, Austria.

Bioinformatics Advances
|November 21, 2025
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Summary

GlobDB consolidates 14 genomic catalogs, significantly expanding microbial species genome representation. This comprehensive database offers consistent taxonomy and annotations for enhanced research accessibility.

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Area of Science:

  • Microbiology
  • Bioinformatics
  • Genomics

Background:

  • Numerous microbial genomes are stored outside standard INSDC databases, hindering comprehensive analysis.
  • A need exists for a unified resource to access and analyze microbial genomic data.

Purpose of the Study:

  • To create a centralized, comprehensive database of microbial genomes.
  • To improve the accessibility and usability of microbial genomic data for research.

Main Methods:

  • Aggregation of 14 independent genomic catalogues.
  • Species-dereplication of microbial genomes.
  • Standardization of taxonomy and annotations across datasets.

Main Results:

  • The GlobDB database was developed, integrating 14 genomic catalogues.
  • GlobDB provides species-dereplicated microbial genomes with consistent taxonomy and annotations.
  • The number of microbial species represented in GlobDB more than doubles that of existing standard databases.

Conclusions:

  • GlobDB offers a significantly expanded and unified resource for microbial genomics.
  • The database enhances research by providing consistent, accessible genomic data.
  • GlobDB is publicly available at https://globdb.org/.