Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Data Validation01:15

Data Validation

557
Method validation is a crucial process in analytical chemistry designed to confirm that a given method consistently produces reliable and high-quality results. This process is essential when a method is applied to different sample matrices or when procedural modifications are made, ensuring that the results meet acceptable standards across various applications.
Key parameters for method validation include:
557

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Bringing 5-FU monitoring home: microsampling as the potential next step beyond DPD deficiency screening.

Expert opinion on drug metabolism & toxicology·2026
Same author

Cotrimoxazole in the intensive care unit: an impossible task for the fixed association in real life?

The Journal of antimicrobial chemotherapy·2025
Same author

The misleading accuracy of propofol PK/PD models: why hypoalbuminemia matters.

Expert opinion on drug metabolism & toxicology·2025
Same author

Acetalax and Bisacodyl for the Treatment of Triple-Negative Breast Cancer: A Combined Molecular and Preclinical Study.

Cancer research communications·2025
Same author

Navigating the complexities of azole antifungal therapy through pharmacokinetic concepts: a case of prolonged isavuconazole toxicity.

The Journal of antimicrobial chemotherapy·2025
Same author

Misleading Renal Function Evaluation Leading to Severe Methotrexate-Induced Toxicity.

Therapeutic drug monitoring·2024
Same journal

CDK4/6 inhibitor-statin interaction and rhabdomyolysis in breast cancer treatment: a case-based systematic review.

Cancer chemotherapy and pharmacology·2026
Same journal

ALK rearrangements and resistance mutations in non-small cell lung cancer: molecular mechanisms and therapeutic implications.

Cancer chemotherapy and pharmacology·2026
Same journal

Central nervous system penetration of imatinib in acute lymphoblastic leukemia: Pharmacokinetic analysis and clinical implications.

Cancer chemotherapy and pharmacology·2026
Same journal

Severe Methotrexate toxicity after ectopic pregnancy: a case report managed with continuous renal replacement therapy.

Cancer chemotherapy and pharmacology·2026
Same journal

Model-based meta-analysis of individual patient data for the characterization of intravenous 5-fluorouracil population pharmacokinetics.

Cancer chemotherapy and pharmacology·2026
Same journal

Fosnetupitant for long-delayed chemotherapy-induced nausea and vomiting in oxaliplatin-based regimens: a prospective observational study (LODEC-N).

Cancer chemotherapy and pharmacology·2026
See all related articles

Related Experiment Video

Updated: Jan 10, 2026

Author Spotlight: Plant Primary Organs Profiling Using 13C6-Glucose Labeling and LC-MS
04:32

Author Spotlight: Plant Primary Organs Profiling Using 13C6-Glucose Labeling and LC-MS

Published on: March 22, 2024

1.2K

Assessing plastic waste associated with LC-MS/MS method validation procedures.

Romain Beziat1,2, Chan Laurent-Britel1, Samuel Huguet1

  • 1Department of Pharmacologie, Institut Curie, PSL Research University, Saint-Cloud, F- 92210, France.

Cancer Chemotherapy and Pharmacology
|November 21, 2025
PubMed
Summary
This summary is machine-generated.

Laboratory diagnostics contribute significantly to climate change. This study quantified plastic waste from validating a tamoxifen LC-MS/MS method, finding daily waste exceeded EU per capita levels, suggesting potential reductions in stability studies.

Keywords:
Bioanalytical method validationGreenhouse gas emissionsPlastic wasteTamoxifen

More Related Videos

Identifying Per- and Polyfluorinated Chemical Species with a Combined Targeted and Non-Targeted-Screening High-Resolution Mass Spectrometry Workflow
09:04

Identifying Per- and Polyfluorinated Chemical Species with a Combined Targeted and Non-Targeted-Screening High-Resolution Mass Spectrometry Workflow

Published on: April 18, 2019

13.0K
Extraction of Organochlorine Pesticides from Plastic Pellets and Plastic Type Analysis
10:12

Extraction of Organochlorine Pesticides from Plastic Pellets and Plastic Type Analysis

Published on: July 1, 2017

12.1K

Related Experiment Videos

Last Updated: Jan 10, 2026

Author Spotlight: Plant Primary Organs Profiling Using 13C6-Glucose Labeling and LC-MS
04:32

Author Spotlight: Plant Primary Organs Profiling Using 13C6-Glucose Labeling and LC-MS

Published on: March 22, 2024

1.2K
Identifying Per- and Polyfluorinated Chemical Species with a Combined Targeted and Non-Targeted-Screening High-Resolution Mass Spectrometry Workflow
09:04

Identifying Per- and Polyfluorinated Chemical Species with a Combined Targeted and Non-Targeted-Screening High-Resolution Mass Spectrometry Workflow

Published on: April 18, 2019

13.0K
Extraction of Organochlorine Pesticides from Plastic Pellets and Plastic Type Analysis
10:12

Extraction of Organochlorine Pesticides from Plastic Pellets and Plastic Type Analysis

Published on: July 1, 2017

12.1K

Area of Science:

  • Environmental Science
  • Clinical Chemistry
  • Pharmaceutical Analysis

Background:

  • The healthcare sector, particularly laboratory diagnostics, is a significant contributor to greenhouse gas emissions (8-10% in France).
  • Method validation processes in diagnostics generate substantial plastic waste, impacting environmental sustainability.

Purpose of the Study:

  • To assess the plastic waste generated during the validation of a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method.
  • To quantify the environmental burden of plastic consumables used in pharmaceutical analysis method validation.

Main Methods:

  • Plastic consumables were collected throughout the LC-MS/MS method validation process.
  • Waste was weighed to determine the total plastic burden, following ICH M10 guidelines.
  • Method performance was evaluated for linearity, accuracy, precision, carry-over, recovery, stability, and robustness.

Main Results:

  • The LC-MS/MS method met EMA/ICH acceptance criteria for linearity, accuracy, precision, and robustness.
  • Plastic waste generated during the 39-day validation totaled 5.7 kg (approximately 146 g/day).
  • This daily waste output was ~1.5 times the average per capita plastic consumption in the EU.

Conclusions:

  • Method validation for tamoxifen and endoxifen monitoring produced significant plastic waste.
  • There is potential to reduce plastic waste by optimizing or eliminating certain stability studies, particularly those under controlled conditions.
  • Sustainable practices in laboratory diagnostics are crucial to mitigate environmental impact.