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Regulation of carbonic anhydrase IX gene expression through the LncRNA TRPM2-AS is associated with tumor progression

Marziye Emamian1, Samane Sharif2,3, Baratali Mashkani4

  • 1Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.

Discover Oncology
|November 21, 2025
PubMed
Abstract

Insights

A specific long non-coding RNA, TRPM2-AS, targets carbonic anhydrase IX (CAIX) in colorectal cancer (CRC). Targeting TRPM2-AS suppresses CAIX, inhibiting CRC cell proliferation and migration, offering a potential therapeutic strategy.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Carbonic anhydrase IX (CAIX) is upregulated in tumors due to hypoxia, promoting cancer progression.
  • Identifying specific molecular targets is crucial for effective colorectal cancer (CRC) therapy.

Purpose of the Study:

  • To identify a long non-coding RNA (lncRNA) that targets CAIX in CRC.
  • To investigate the therapeutic potential of targeting this lncRNA in CRC cells.

Main Methods:

  • Bioinformatic analysis identified CAIX-associated lncRNAs.
  • Quantitative real-time PCR (qRT-PCR) measured lncRNA-TRPM2-AS and CAIX expression in CRC tissues.
  • Antisense Oligonucleotide (ASO) technology was used to block lncRNA-TRPM2-AS in HCT116 cells.
  • Cell proliferation, apoptosis, migration, and invasion assays assessed cellular behavior.

Main Results:

  • LncRNA-TRPM2-AS and CAIX were significantly overexpressed in CRC tissues, particularly in stages II/III.
  • Blocking lncRNA-TRPM2-AS reduced CAIX expression by approximately 50% in HCT116 cells.
  • Inhibition of lncRNA-TRPM2-AS suppressed cell proliferation and migration while increasing apoptosis.

Conclusions:

  • LncRNA-TRPM2-AS plays a key role in regulating CAIX expression and hypoxia in CRC.
  • Targeting lncRNA-TRPM2-AS offers a promising therapeutic strategy to down-regulate CAIX and combat CRC progression and drug resistance.
Keywords:
Antisense oligonucleotide (ASO)CRCCarbonic anhydrase IX (CAIX)HypoxiaLncRNA TRPM2-AS

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